Bad [p Ser112] Antibody - BSA Free

Name: Bad [p Ser112] Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP3-23435
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23435

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all Bad Primary Antibodies »

Product Specifications

Immunogen

Phospho-Bad (Ser-112) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 112 in mouse Bad. This peptide sequence is highly conserved in human (Ser-75) and rat (Ser-113) Bad. [UniProt# Q61337]

Modification

p Ser112

Specificity

The antibody detects a 23 kDa doublet corresponding to the apparent molecular mass of phosphorylated Bad on SDS-PAGE immunoblots of mouse J774A.1 treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for Bad [p Ser112] Antibody - BSA Free

Western Blot: Bad [p Ser112] Antibody [NBP3-23435] -

Western Blot: Bad [p Ser112] Antibody [NBP3-23435] - Western blot analysis of mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Bad (lanes 1 & 2), and rabbit polyclonal anti-Bad (Ser-112) (lanes 3 & 4).

Applications for Bad [p Ser112] Antibody - BSA Free

Application

Recommended Usage

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bad

Bad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xL over Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse the death repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activated Bad translocates to the mitochondria and displace Bcl-2 from tBid sequestration to initiate mitochondrial dysfunction. The switching on/off of its phosphorylation by growth/survival factors regulates Bad activity.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Alternate Names

BBC6, BCL2L8

Gene Symbol

BAD

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Product Specific Notices for Bad [p Ser112] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.