Bafilomycin A1

Tocris Bioscience | Catalog # 1334

H+-ATPase (vacuolar) inhibitor
Tocris Bioscience

Key Product Details

Description

H+-ATPase (vacuolar) inhibitor
View all H+-ATPase Inhibitors »

Product Description

Bafilomycin A1 is a highly potent, selective inhibitor of vacuolar H+-ATPases (IC50 = 0.6 - 1.5 nM in bovine chromaffin granules). Selective for v-ATPase over other ATP hydrolyzing enzymes such as F-ATPases and the H+/K+-ATPase (P-ATPase). Thought to inhibit autophagy either by blocking autophagosome-lysosome fusion (in H4IIE cells), or by blocking lysosomal degradation. Bafilomycin A1 accelerates wound healing in db/db mice. Low dose Bafilomycin A1 attenuates patient-derived CD34+CD19+ leukemia stem cells (LSC) and inhibits LSC proliferation in an animal model. Bafilomycin A1 impairs Zika virus infection in vitro.

Product Specifications for Bafilomycin A1

Molecular Weight

622.84

Formula

C35H58O9

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

(3Z,5E,7R,8S,9S,11E,13E,15S,16R)-8-Hydroxy-16-[(1S,2R,3S)-2-hydroxy-1-methyl-3-[(2R,4R,5S,6R)-tetrahydro-2,4-dihydroxy-5-methyl-6-(1-methylethyl)-2H-pyran-2-yl]butyl]-3,15-dimethoxy-5,7,9,11-tetramethyloxacyclohexadeca-3,5,11,13-tetraen-2-one

CAS Number

88899-55-2

PubChem ID

72311

InChI Key

WEEFNMFMNMASJY-UHFFFAOYSA-M

SMILES

O[C@H]([C@H](C)/C=C(C)/C=C1OC)[C@@H](C)C/C(C)=C/C=C\[C@H](OC)C([C@@H](C)[C@H]([C@@H]([C@@]2(O)C[C@@H](O)[C@H](C)[C@@H]([C@H](C)C)O2)C)O)OC/1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 4.98 8

Preparing Stock Solutions for Bafilomycin A1

The following data is based on the product molecular weight 622.84.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.08 mM 20.07 mL 100.35 mL 200.69 mL
0.4 mM 4.01 mL 20.07 mL 40.14 mL
0.8 mM 2.01 mL 10.03 mL 20.07 mL
4 mM 0.40 mL 2.01 mL 4.01 mL

Calculators

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Product Documents for Bafilomycin A1

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Bafilomycin A1

For research use only

Related Research Areas

Autophagy

Customer Reviews for Bafilomycin A1 (3)

4.3 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
33%
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1 Stars
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  • test
    Name: Xiaogang Wang
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Human Macrophages
    Verified Customer | Posted 06/24/2021
    We used BafA inhibitor to investigate the autophagy induced by bacterial toxins. To confirm whether BafA1 works, human macrophages were treated with or without 100 nM BafA for 1 h, and the levels of LC3-I and LC3-II within human macrophages were determined by western blot using LC3 antibody. As expected (shown in figure), treatment of cells with BafA1 resulted in an increased level of LC3-I and LC3-II, suggesting that the fusion of autophagosomes and lysosomes was blocked and resulted in the accumulation of autophagosomes.
    Bafilomycin A1 1334
  • BAF
    Name: shubham dubey
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: H9C2
    Verified Customer | Posted 05/28/2021
    Treated H9C2 cells to check the autophagy.
    Bafilomycin A1 1334
  • Good autophagy inhibitor
    Name: JING HE
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HT29
    Verified Customer | Posted 03/27/2020
    HT29 cells transfected with ad-mRFP-GFP-LC3 adenovirus after incubation with or without LPS and Baf A1 at different time points.
    Bafilomycin A1 1334

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