BLIMP1/PRDM1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-27180

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Bovine

Cited:

Bovine

Predicted:

Canine (100%), Mouse (94%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Amino acids 692-719 of human BLIMP-1 (PRDM1) were used as the immunogen for this antibody.

Reactivity Notes

The BLIMP-1 (PRDM1) amino acid sequence used as immunogen for this antibody is 85% homologous in mouse.

Marker

Plasma Cell Marker

Specificity

It is 100% homologous in isoforms 1 and 2 of human BLIMP-1 (PRDM1).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BLIMP1/PRDM1 Antibody - BSA Free

Western Blot: BLIMP1/PRDM1 Antibody [NBP2-27180]

Western Blot: BLIMP1/PRDM1 Antibody [NBP2-27180]

Western Blot: BLIMP1/PRDM1 Antibody [NBP2-27180] - Analysis of BLIMP-1 using BLIMP-1 polyclonal antibody in cattle skin extracts from 5 different animals. Protein expression of increased 1.5-4 fold when measured 24 h after tick infection. (loading was standardized with a GAPDH antibody). BLIMP-1 was detected at ~88 kDa. Data courtesty of Kongsuwan et al.

Applications for BLIMP1/PRDM1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole sera with 50% Glycerol

Format

BSA Free

Preservative

0.025% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BLIMP1/PRDM1

An 88kDa transcription repressor protein is a human homolog of mouse Blimp-1. It belongs to the family of PRDM family and contains a PR domain and 5 kruppel Like-zinc finger motifs at the C-terminal region. BLIMP-1 plays a pivotal role in inducing and regulating terminal differentiation of B-lymphocytes. It recruits HDAC and complex of Groucho/TLE family proteins to the DNA and actively represses vital genes required for B-cell proliferation including c-myc, CIITA promoter III, CD23b and PAX-5 promoter. BLIMP-1 silences IFNi -gene expression by binding specifically to PRDI of the IFNi gene promoter. It is continuously expressed in all Plasma cells, but is not detected in early bone marrow B-cells, memory B-cells in spleen and most germinal center B-cells. In myeloma cells, PRDI-BF1i, a truncated isoform of BLIMP-1 is expressed that has an impaired ability to repress transcription suggesting a clinical implication.

Long Name

B Lymphocyte Induced Maturation Protein

Alternate Names

PRDM1, ZNFPR1A1

Gene Symbol

PRDM1

Additional BLIMP1/PRDM1 Products

Product Documents for BLIMP1/PRDM1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BLIMP1/PRDM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BLIMP1/PRDM1 Antibody - BSA Free

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FAQs for BLIMP1/PRDM1 Antibody - BSA Free

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  • Q: I would like to use this antibody for western blotting. Is it recommended for WB test?

    A: This antibody has been validated for Western blotting with samples from human and bovine, and I can confirm that the immunogen sequence for NBP2-27180 is 100% homologous in human, chimp, dog and bovine, 94% homologous in mouse, and 88% homologous in chicken, zebrafish and rat. As such, the antibody would be expected to react with zebrafish Blimp-1, although we do not currently have any testing data from zebrafish that I can share with you.

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