C. botulinum BoNT-D Heavy Chain Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB6639U Datasheet / COA / SDS

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FAB6639U-100UG

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C. botulinum BoNT-D Heavy Chain Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

C. botulinum

Specificity

Detects recombinant C. botulinum BoNT-D Heavy Chain in direct ELISAs and Western blots. 100% and approximately 10% cross-reactivity with recombinant C. botulinum BoNT-C1 Heavy Chain is observed in Western blots and direct ELISAs, respectively. In direct E

Source

Monoclonal Mouse IgG2b Clone # 682810

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant Clostridium botulinum Neurotoxin Type D Heavy Chain
Asn862-Glu1276
Accession # P19321

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BoNT-D Heavy Chain

BoNT-D (Botulinum neurotoxin serotype D) is produced by Clostridium botulinum and inhibits acetylcholine release from neuromuscular junctions. Notably, BoNT-D is not toxic to human cells. The 1276 amino acid (aa) BoNT-D precursor is cleaved to generate a disulfide-linked dimer of the 50‑55 kDa light chain peptidase and the 100 kDa heavy chain. The heavy chain contains receptor binding and pore forming domains. The light chain translocates through this pore to the target cell cytosol where it cleaves synaptobrevin, thus blocking synaptic vesicle fusion with the presynaptic membrane. Within aa 862-1276 of the heavy chain, BoNT-D shares 31%, 33%, and 41% aa sequence identity with BoNT-A, -B, and -C1 heavy chains, respectively.

Long Name

Botulinum Neurotoxin Type D Heavy Chain

Alternate Names

BoNTD Heavy Chain; BoNT-D Heavy Chain