C. botulinum BoNT-E Heavy Chain Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB7135V Datasheet / COA / SDS

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FAB7135V-100UG

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C. botulinum BoNT-E Heavy Chain Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

C. botulinum

Specificity

Detects C. botulinum BoNT-E Heavy Chain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant Heavy Chains from BoNT-A or BoNT-G is observed.

Source

Monoclonal Mouse IgG2b Clone # 785208

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant C. botulinum BoNT-E Heavy Chain
Lys845-Lys1251
Accession # P30995

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BoNT-E Heavy Chain

Botulinum Neurotoxin Type E is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2‑4). E. coli-expressed recombinant light chains are active proteases. In the absence of the heavy chains, however, they lack toxicity because they cannot enter into host cells.

Long Name

Botulinum Neurotoxin Type E Heavy Chain

Alternate Names

BoNTE Heavy Chain; BoNT-E Heavy Chain; Bontoxilysin-E