C. tetani TeNT Light Chain Antibody (604023)

R&D Systems | Catalog # MAB6535

R&D Systems

Key Product Details

Species Reactivity

Validated:

C. tetani

Cited:

Bacteria - Clostridium tetani

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 604023
View all TeNT Light Chain Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant C. tetani TeNT Light Chain
Pro2-Gly430
Accession # P04958

Specificity

Detects TeNT Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant TeNT Heavy Chain is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for C. tetani TeNT Light Chain Antibody (604023)

Detection ofC. tetaniTeNT Light Chain antibody by Western Blot.

Detection ofC. tetaniTeNT Light Chain by Western Blot.

Western blot shows recombinantC. tetaniTeNT Light Chain. PVDF Membrane was probed with 2 µg/mL ofC. tetaniTeNT Light Chain Monoclonal Antibody (Catalog # MAB6535) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TeNT Light Chain at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for C. tetani TeNT Light Chain Antibody (604023)

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with recombinant C. tetani TeNT Light Chain, see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: Recombinant C. tetani TeNT Light Chain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TeNT Light Chain

Tetanus toxin, a member of the peptidase M27 family of proteins, is produced by the anaerobic spore-forming bacteria Clostridium tetani as a single-chain polypeptide that is 1315 amino acids (aa) in length.  The protein is subsequently cleaved by an endogenous protease to yield the 52 kDa tetanus toxin light chain (TeNT-LC), which is 457 aa in length, and the 98 kDa tetanus toxin heavy chain that is 858 aa long.  The light and heavy chains are linked by a disulfide bridge and are non-toxic after separation.  Tetanus toxin acts by inhibiting neurotransmitter release.  It binds to peripheral neural synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons.  It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the ’76-Gln-|-Phe-77’ bond of synaptobrevin-2. 

Long Name

Tetanus Neurotoxin Type Light Chain

Alternate Names

TeNT-LC, Tentoxylysin, tetX

Entrez Gene IDs

1061100 (C. tetani)

Gene Symbol

TETX

UniProt

P04958 (C. tetani)

Additional TeNT Light Chain Products

Product Documents for C. tetani TeNT Light Chain Antibody (604023)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for C. tetani TeNT Light Chain Antibody (604023)

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for C. tetani TeNT Light Chain Antibody (604023)

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