Calsenilin Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-00359

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Calsenilin Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human Calsenilin (Uniprot#: Q9Y2W7-1; Met1-Ile256)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Calsenilin Antibody - Azide and BSA Free

Calsenilin Antibody - Azide and BSA Free

Immunoprecipitation: Calsenilin Antibody [NBP3-00359] -

Immunoprecipitation: Calsenilin Antibody [NBP3-00359] - KCNIP3 was immunoprecipitated using: Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg THP-1 Whole Cell Lysate 0.5 uL NBP3-00359 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00359, at 1:350 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 35 kDa
Calsenilin Antibody - Azide and BSA Free

Western Blot: Calsenilin Antibody [NBP3-00359] -

Western Blot: Calsenilin Antibody [NBP3-00359] - NBP3-00359 at 1:500 dilution Lane A: Mouse heart Tissue Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:36 kDa

Applications for Calsenilin Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calsenilin

The human prodynorphin gene, which is involved in memory acquisition and pain, is regulated by its downstream regulatory element (DRE). The DRE acts as a location-dependent gene silencer. DREAM is a transcriptional repressor, DRE-antagonist modulator. It is the first known Ca2+ binding protein that binds to DNA and regulates transcription.

Alternate Names

calsenilin, calsenilin, presenilin-binding protein, EF hand transcription factor, CSEN MGC18289, DREAM, DREAMA-type potassium channel modulatory protein 3, KChIP3, KCHIP3 DRE-antagonist modulator, Kv channel interacting protein 3, calsenilin, Kv channel-interacting protein 3, potassium channel interacting protein 3

Gene Symbol

KCNIP3

Additional Calsenilin Products

Product Documents for Calsenilin Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Calsenilin Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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