Catenin alpha 1 [p Tyr148] Antibody

Novus Biologicals | Catalog # NBP3-23196

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Catenin alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Phospho-Catenin alpha 1 (Tyr-148) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 148 in human Catenin alpha 1. This peptide sequence is highly conserved in rat and mouse Catenin alpha 1, but is not conserved in Catenin alpha 2 or Catenin alpha 3. [UniProt# P35221]

Modification

p Tyr148

Specificity

This antibody was cross-adsorbed to unrelated phospho-tyrosine peptide before affinity purification using phospho-Catenin alpha 1 (Tyr-148) peptide (without carrier). The antibody detects a 102 kDa protein corresponding to the molecular mass of a1-Catenin on SDS-PAGE immunoblots of rat PC12 cells treated with pervanadate.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Catenin alpha 1 [p Tyr148] Antibody

Catenin alpha 1 [p Tyr148] Antibody

Western Blot: Catenin alpha 1 [p Tyr148] Antibody [NBP3-23196] -

Western Blot: Catenin alpha 1 [p Tyr148] Antibody [NBP3-23196] - Western blot analysis of rat PC12 cells treated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti- -Catenin monoclonal (lanes 1 & 2), anti-Catenin alpha 1 (Tyr-148) phospho-specific (lanes 3 & 4), or anti-Catenin alpha 1 (a.a. 143-153) (lanes 5 & 6).

Applications for Catenin alpha 1 [p Tyr148] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Catenin alpha 1

Cadherin and catenin compose cell adhesion complex and are indispensable for tight cell-cell adhesion. Dysfunction of this adhesion complex causes dissociation of cancer cells from primary tumor nodules, thus possibly contributing to cancer invasion and metastasis (1). At least three proteins (alpha, beta, and gamma catenin) comprise the cytoplasmic domain of the cadherin cell-cell adhesion complex. Data, with the reported structure of other catenin genes, suggest that vinculin and alpha-catenin generate a superfamily of proteins mediating membrane-cytoskeletal associations (2). Presenilin-1 (PS1) overexpression in human kidney cells enhances cell-cell adhesion and data show that PS1 incorporates into the cadherin/catenin adhesion system and regulates cell-cell adhesion. PS1 concentrates at intercellular contacts in epithelial tissue; in brain, it forms complexes with both E- and N-cadherin and concentrates at synaptic adhesions (3).

Alternate Names

Alpha E-catenin, alpha-catenin, alpha-E-catenin, alphaE-catenin, Cadherin-associated protein, CAP102, catenin (cadherin-associated protein), alpha 1 (102kD), catenin (cadherin-associated protein), alpha 1, 102kDa, catenin alpha-1, FLJ36832, FLJ52416, Renal carcinoma antigen NY-REN-13

Gene Symbol

CTNNA1

Additional Catenin alpha 1 Products

Product Documents for Catenin alpha 1 [p Tyr148] Antibody

Certificate of Analysis

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Product Specific Notices for Catenin alpha 1 [p Tyr148] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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