Key Product Details

Species Reactivity

Canine

Applications

Neutralization

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 676117
View all CCL5/RANTES Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant canine CCL5/RANTES
Ser24-Ser91
Accession # Q8HYS0

Specificity

Detects canine CCL5/RANTES in direct ELISAs. In Western blots, no cross-reactivity with recombinant cotton rat CCL5, recombinant feline CCL5, recombinant human (rh) CCL5, 17, 18, recombinant mouse (rm) CCL5, 7, or 18 is observed. In direct ELISAs, approximately 25% cross‑reactivity with recombinant human CCL5 and no cross-reactivity with recombinant CCL5 from cotton rat, cat, or mouse is observed. No cross-reactivity with rhCCL1, 4, 11, 14, 16, 21, 23, 24, 26, 27, rmCCL6, 11, 21, 24, 26, or 27 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for CCL5/RANTES Antibody (676117) [PE]

Application
Recommended Usage

Neutralization

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB6045. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CCL5/RANTES

RANTES (Regulated-on-activation-normal-T-cell-expressed-and-secreted) is a member of the "intercrine/chemokine" family of cytokines, has been previously shown to be chemotactic for monocytes and T cells of memory/helper phenotype. RANTES has been shown to cause the release of histamine from basophils and activates eosinophils. It also binds to CCR1, CCR3, CCR4 and CCR5 (1). This CC-chemokine enhances antigen-specific T helper (Th) type-1 responses against HIV-1 (2). CC chemokines are small inducible proteins that are related to interleukin 8. In lung adenocarcinoma, RANTES is a predictor of survival while that of interleukin (IL)-8 is associated with a poor prognosis. In several models, tumorigenesis is abolished by RANTES, while it is facilitated by IL-8. Site-directed mutagenesis indicated that regulation of RANTES promoter activity required two nuclear factor (NF)-kappaB response elements but not its activator protein (AP)-1 binding sites (3).

Alternate Names

RANTES, SISd

Gene Symbol

CCL5

Additional CCL5/RANTES Products

Product Documents for CCL5/RANTES Antibody (676117) [PE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CCL5/RANTES Antibody (676117) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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