Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all CD3 delta/CD3d Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human CD3 delta (Accession#: NP_000723.1; Met 1-Ala 105)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD3 delta Antibody - Azide and BSA Free

Western Blot: CD3 delta Antibody [NBP2-99673]

Western Blot: CD3 delta Antibody [NBP2-99673]

Western Blot: CD3 delta Antibody [NBP2-99673] - Anti-CD3 delta rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: Hu-T78 Whole Cell Lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 21 kDa
Immunoprecipitation: CD3 delta Antibody [NBP2-99673]

Immunoprecipitation: CD3 delta Antibody [NBP2-99673]

Immunoprecipitation: CD3 delta Antibody [NBP2-99673] - CD3 delta was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HuT78 Whole Cell Lysate. 0.5 ul anti-CD3 delta rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CD3 delta rabbit polyclonal antibody, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa

Applications for CD3 delta Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 delta/CD3d

CD3 delta is encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq]

Long Name

CD3 delta

Alternate Names

CD3D

Gene Symbol

CD3D

Additional CD3 delta/CD3d Products

Product Documents for CD3 delta Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD3 delta Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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