CD40 Ligand/TNFSF5

Novus Biologicals | Catalog # DDX-S2

Novus Biologicals
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Key Product Details

Applications

Flow Cytometry, Functional Assay, Immunocytochemistry/ Immunofluorescence

Product Specifications

Host

Horse

Application Notes

Murine L cells were stably transfected with human CD40L (L6) and freeze-dried through proprietary process. These cells are primarily intended to activate human cells through triggering of CD40 surface antigen but were also demonstrated to activate B cells from several animal species. L6 cells successfully support the differentiation of human monocyte towards the dendritic cell lineage. L6 cells are included in the Human Blood B booster and the Animal Blood B Booster kits, and support B cell growth and Ig secretion, but can also used alone for transient cultures.

Reactivity Notes

Rat, Sheep, Dog, Cat, Pig, Horse, Rabbit

Scientific Data Images for CD40 Ligand/TNFSF5

Immunocytochemistry: CD40 Ligand/TNFSF5 [DDX-S2]

Immunocytochemistry: CD40 Ligand/TNFSF5 [DDX-S2]

Immunocytochemistry: CD40 Ligand/TNFSF5 Lysate [DDX-S2] - PBMC culturedin the presence of DDX-S2
Flow Cytometry: CD40 Ligand/TNFSF5 [DDX-S2]

Flow Cytometry: CD40 Ligand/TNFSF5 [DDX-S2]

Flow Cytometry: CD40 Ligand/TNFSF5 Lysate [DDX-S2] - Surface cytometry analysis of CD40L expression
Immunofluorescence: CD40 Ligand/TNFSF5 [DDX-S2]

Immunofluorescence: CD40 Ligand/TNFSF5 [DDX-S2]

Immunofluorescence: CD40 Ligand/TNFSF5 Lysate [DDX-S2] - IF stainingof L6 cells
In vitro assay: CD40 Ligand/TNFSF5 [DDX-S2]

In vitro assay: CD40 Ligand/TNFSF5 [DDX-S2]

In vitro assay: CD40 Ligand/TNFSF5 Lysate [DDX-S2]
CD40 Ligand/TNFSF5 Lysate [DDX-S2] - Freeze-dried L6 cells are part of the Blood B Booster technology intended to immortalizehuman B cells and to activate animal B cells

Formulation, Preparation, and Storage

Preparation Method

Resuspended L6 cells can be kept at 4C under sterilized conditions for up to 2 months.

Preservative

No Preservative

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Protect from light. Store at -20C. Avoid freeze-thaw cycles. After opening store under sterile conditions.

Background: CD40 Ligand/TNFSF5

CD40L is the ligand for CD40, a member of the tumor necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L.

Alternate Names

CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP

Gene Symbol

CD40LG

Additional CD40 Ligand/TNFSF5 Products

Product Documents for CD40 Ligand/TNFSF5

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD40 Ligand/TNFSF5

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD40 Ligand/TNFSF5

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    • Q: I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
      A: Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
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