CHD1 Antibody

Novus Biologicals | Catalog # NB100-60411

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Chicken (100%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Knockdown Validated

Cited:

Western Blot, Immunoprecipitation, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CHD1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1660 and 1710 of human chromodomain helicase DNA binding protein 1 using the numbering given in entry NP_001261.2 (GeneID 1105).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 20161795).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Knockout (KO) Validated Rabbit CHD1 Antibody (NB100-60411) is a polyclonal antibody validated for use in WB, IP and ChIP. Anti-CHD1 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CHD1 Antibody

Knockout Validated: CHD1 Antibody [NB100-60411]

Western Blot: CHD1 Antibody [NB100-60411]

Western Blot: CHD1 Antibody [NB100-60411] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human CHD1 Polyclonal Antibody (Catalog # NB100-60411) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CHD1 at approximately 260 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Western Blot: CHD1 Antibody [NB100-60411]

Western Blot: CHD1 Antibody [NB100-60411]

Western Blot: CHD1 Antibody [NB100-60411] - Detection of Human CHD1 on HeLa whole cell lysate using NB100-60411. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody NB100-60410.
Chromatin Immunoprecipitation Sequencing: CHD1 Antibody [NB100-60411] - Localization of CHD1 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CHD1 antibody NB100-60411 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CHD1 binding within a 3.5 Mb region of the human X chromosome as detected using anti-CHD1 antibody NB100-60411. ChIP-seq validation performed by Diogenode, Denville, NJ.
Chromatin Immunoprecipitation: CHD1 Antibody [NB100-60411] - ChIP-chip scatter plot of anti-CHD1 (NB100-60411) enriched DNA binding sites versus input reference DNA. CHD1 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-CHD1 ChIP, normal rabbit IgG showed little enrichment.
Knockdown Validated: CHD1 Antibody [NB100-60411]

Immunocytochemistry/ Immunofluorescence: CHD1 Antibody [NB100-60411]

CHD1-Antibody-Knockdown-Validated-NB100-60411-img0008.jpg

Applications for CHD1 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1-5 ug/mg lysate

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
WB in human was reported in the following publication: PMID: 21880597

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CHD1

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

Long Name

Chromodomain Helicase DNA Binding Protein 1

Alternate Names

ATP-dependent helicase CHD1, CHD-1, chromodomain helicase DNA binding protein 1, chromodomain-helicase-DNA-binding protein 1, DKFZp686E2337, EC 3.6.1, EC 3.6.4.12

Entrez Gene IDs

1105 (Human)

Gene Symbol

CHD1

UniProt

O14646 (Human)

Additional CHD1 Products

Product Documents for CHD1 Antibody

Certificate of Analysis

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Product Specific Notices for CHD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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