CHD4 Antibody

Novus Biologicals | Catalog # NB100-57521

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CHD4 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1625 and 1675 of human chromodomain helicase DNA binding protein 4 (Mi2 autoantigen 218 kDa protein) using the numbering given in entry NP_001264.2 (GeneID 1108).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHD4 Antibody

Western Blot: CHD4 Antibody [NB100-57521]

Western Blot: CHD4 Antibody [NB100-57521]

Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.
Immunoprecipitation: CHD4 Antibody [NB100-57521]

Immunoprecipitation: CHD4 Antibody [NB100-57521]

Immunoprecipitation: CHD4 Antibody [NB100-57521] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57521 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57519, NB100-57520), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). Detection: Chemiluminescence with an exposure time of 1 second.

Applications for CHD4 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in CHIP reported in scientific literature (PMID:31934863).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CHD4

CHD4/Mi2beta is a helicase component of the nucleosome remodeling and deacetylase (NuRD) complex that functions to remodel chromatin and repress transcription. Outside of the NuRD complex, CHD4/Mi2beta can function as an activator of transcription in association with p300 histone acetyltransferase. Alternate names for CHD4/Mi2beta include chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b, and DKFZp686E06161.

Alternate Names

ATP-dependent helicase CHD4, CHD-4, chromodomain helicase DNA binding protein 4, chromodomain-helicase-DNA-binding protein 4, DKFZp686E06161, EC 3.6.1, EC 3.6.4.12, Mi-2 autoantigen 218 kDa protein, Mi-2b, Mi2-BETA

Entrez Gene IDs

1108 (Human); 107932 (Mouse)

Gene Symbol

CHD4

UniProt

Q14839 (Human)

Additional CHD4 Products

Product Documents for CHD4 Antibody

Certificate of Analysis

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Product Specific Notices for CHD4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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