Cholesterol Oxidase Antibody - BSA Free

Novus Biologicals | Catalog # NB600-625

Novus Biologicals
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Key Product Details

Species Reactivity

Bacteria

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Cholesterol Oxidase Primary Antibodies »

Product Specifications

Immunogen

Cholesterol Oxidase [Streptomyces] (Uniprot: P12676)

Reactivity Notes

Reactive with Cholesterol Oxidase from Streptomyces.
Cross reactivity against Cholesterol Oxidase from other sources is unknown

Specificity

Cholesterol Oxidase

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Cholesterol Oxidase Antibody - BSA Free

Western Blot: Cholesterol Oxidase Antibody [NB600-625]

Western Blot: Cholesterol Oxidase Antibody [NB600-625]

Western Blot: Cholesterol Oxidase Antibody [NB600-625] - Analysis using HRP conjugate of NB600-625. Detection of purified N-acylmanosamino-1-Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat.
Western Blot: Cholesterol Oxidase Antibody [NB600-625]

Western Blot: Cholesterol Oxidase Antibody [NB600-625]

Western Blot: Cholesterol Oxidase Antibody [NB600-625] - Detection of purified cholesterol oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified cholesterol oxidase contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with a 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat.
Cholesterol Oxidase Antibody

Cholesterol Oxidase Antibody

Goat anti Cholesterol oxidase antibody ( lot 6534) was used to detect purified cholesterol oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified cholesterol oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with a 1:3000 dilution of primary antibody (ON 4 C in ). Detection shown was using Dylight 488 conjugated Donkey anti goat ( lot 21094 1:10K in TBS/ 1 hr RT). Images were collected using the BioRad VersaDoc System.

Applications for Cholesterol Oxidase Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunoprecipitation

1:100

Western Blot

1:500-1:5000
Application Notes
This product has been tested by western blot. This product is suitable to be assayed against 1.0 ug of Cholesterol Oxidase [Microorganism] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cholesterol Oxidase

Cholesterol Oxidases exist as both type I and type II oxidases and are implicated in bacterial pathogenesis. In addition, they are important as clinical reagents, potential larvicides, and tools in cell biology.

Alternate Names

choA, choD, choM, choS, COD

UniProt

P12676 (Human)

Additional Cholesterol Oxidase Products

Product Documents for Cholesterol Oxidase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cholesterol Oxidase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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