Claudin-5 Antibody (HL3156)

Name: Claudin-5 Antibody (HL3156)
Brand: Novus Biologicals
SKU: NBP3-48773
Price: 529 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-48773

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3156

View all Claudin-5 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide of human Claudin-5.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Claudin-5 Antibody (HL3156)

Immunohistochemistry-Paraffin: Claudin-5 Antibody (HL3156) [NBP3-48773] -

Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:3200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Immunohistochemistry-Paraffin: Claudin-5 Antibody (HL3156) [NBP3-48773] -

Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:2000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Immunohistochemistry-Paraffin: Claudin-5 Antibody (HL3156) [NBP3-48773] -

Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:3200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Immunohistochemistry-Paraffin: Claudin-5 Antibody (HL3156) [NBP3-48773] -

Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:2000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Western Blot: Claudin-5 Antibody (HL3156) [NBP3-48773] -

Unboiled various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Western Blot: Claudin-5 Antibody (HL3156) [NBP3-48773] -

Unboiled mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Claudin-5 Antibody (HL3156) [NBP3-48773] -

Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:2000.
Red: beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Immunohistochemistry-Paraffin: Claudin-5 Antibody (HL3156) [NBP3-48773] -

Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:2000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

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Applications for Claudin-5 Antibody (HL3156)

Application

Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Claudin-5

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specific regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-5 is expressed in the endothelial junctions of the rat liver and in junctions of acinar cells of the pancreas. Human claudin-5 is abundantly expressed in adult lung, heart and skeletal muscle and is deleted in patients with velocardiofacial syndrome, which is characterized by cleft palate, facial dysmorphology and conotruncal heart defects.The human claudin-5 gene maps to chromosome 22q11.21.

Alternate Names

AWAL, BEC1, Claudin5, CLDN5, CPETRL1, TMDVCF, TMVCF1

Gene Symbol

CLDN5

Additional Claudin-5 Products

Product Documents for Claudin-5 Antibody (HL3156)

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Certificate of Analysis

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Product Specific Notices for Claudin-5 Antibody (HL3156)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.