Clozapine N-oxide (CNO)

Tocris Bioscience | Catalog # 4936

Activator of hM3Dq and hM4Di DREADDs
Tocris Bioscience

Key Product Details

Description

Activator of hM3Dq and hM4Di DREADDs

Alternative Names

CNO
View all DREADD Activators »

Product Description

Clozapine N-oxide is a synthetic ligand for human muscarinic engineered receptors, designer receptors activated by designer drugs (DREADD). Binds and activates hM3Dq and hM4Di DREADDs in vitro and in vivo. Metabolite of Clozapine (Cat. No. 0444). Shown to be a P-glycoprotein (P-gp) efflux pump substrate.

Example Applications of Clozapine N-oxide (CNO):

  • Silences hippocampal neurons expressing hM4Di DREADDs in vitro
  • Inhibits locomotor activity of mice expressing hM3Dq in GABAergic VTA neurons
  • Inhibits short term memory retrieval in mice expressing hM4Di in hippocampal neurons
  • Induces heat and mechanical hypersensitivity in mice expressing hM4Di in enkephalinergic neurons in the right lumbar dorsal horn
  • Inhibits markers of social aggression in mice expressing hM4Di in hippocampal CA2 neurons

Alternative potent and selective hM3Dq and hM4Di DREADD agonists available: Deschloroclozapine and DREADD agonist 21

Water soluble salt also available.

This product contains 1 molar equivalent of ethanol.

Product Specifications for Clozapine N-oxide (CNO)

Molecular Weight

342.82

Formula

C18H19ClN4O

Storage

Store at RT

Purity

≥98% (HPLC)

Chemical Name

8-Chloro-11-(4-methyl-4-oxido-1-piperazinyl)-5H-dibenzo[b,e][1,4]diazepine

CAS Number

34233-69-7

PubChem ID

2819

InChI Key

WYRDWWAASBTJLM-UHFFFAOYSA-N

SMILES

ClC(C=C3)=CC1=C3NC(C=CC=C2)=C2C(N4CC[N+](C)([O-])CC4)=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 6.86 20

Preparing Stock Solutions for Clozapine N-oxide (CNO)

The following data is based on the product molecular weight 342.82.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 14.58 mL 72.92 mL 145.85 mL
1 mM 2.92 mL 14.58 mL 29.17 mL
2 mM 1.46 mL 7.29 mL 14.58 mL
10 mM 0.29 mL 1.46 mL 2.92 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 514 publications citing the usage of this product.

Product Documents for Clozapine N-oxide (CNO)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Clozapine N-oxide (CNO)

For research use only

Citations for Clozapine N-oxide (CNO)

Customer Reviews for Clozapine N-oxide (CNO) (8)

4.6 out of 5
8 Customer Ratings
5 Stars
63%
4 Stars
38%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 的 8 reviews Showing All
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  • Good product
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vivo
    Cell Line/Tissue: neuron
    Verified Customer | Posted 06/15/2020
    1 mg/kg
    Clozapine N-oxide (CNO) 4936
  • Highly soluble and stable
    Name: Anonymous
    Species: Mouse
    Assay Type: Ex Vivo
    Verified Customer | Posted 01/04/2018
    Soluble in 0.9% saline. Perfused in brain slice experiment with patch clamp study.
    Clozapine N-oxide (CNO) 4936
  • CNO in DREADD pain targeted Mice
    Name: Anonymous
    Verified Customer | Posted 12/15/2017
    Product provides publishable work, no issues arose when dealing with the substance.
    CNO was used in pair with DREADD mice for a pain related experiment to determine whether inhibiting PBN neurons will attenuate pain reponses. Publication under second revision for Cell.
  • CNO in concert with DREADDs
    Name: Anonymous
    Verified Customer | Posted 12/01/2017
    I injected clozapine N-oxide in rats (IP, 1 mg/ml/kg). The rats had previously underwent stereotaxic surgery in which a virus carrying a gene for a Gi DREADD was infused unilaterally into the dorsal striatum.
  • CNO for chronic DREADD activation
    Name: Anonymous
    Assay Type: In Vivo
    Verified Customer | Posted 12/01/2017
    Added saccharine to counteract bitter taste of DMSO used to dissolve CNO
    In vivo chemogenetic experiments - CNO was added to the drinking water. While there are issues with the back conversion of CNO to clozapine, this is not the manufacturer's problem. CNO experiments still generate important data as long as they utilize proper controls
    Clozapine N-oxide (CNO) 4936
  • Does what it is meant to!
    Name: Anonymous
    Assay Type: In Vivo
    Verified Customer | Posted 10/20/2017
    We use it as both injectable pass the blood brain barrier ICV, and IP injections. We are looking at both behavioral concern and brain regional efficacy in regard to DREADD. So far, this product has been selectively turning on/off our target cells.
  • tail bleed
    Name: Anonymous
    Assay Type: Ex Vivo
    Verified Customer | Posted 09/07/2017
    bleed assay
  • neuron silencer
    Name: Anonymous
    Assay Type: In Vivo
    Verified Customer | Posted 07/17/2017
    neuron silencer

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