Q: NBP2-60620 can this product be used to deteremin C5a?

A: This Human Complement C5 ELISA kit is specifically designed to detect the uncleaved C5. There is no guarantee that it can pick up the signal from either C5a or C5b once C5 is cleaved.

Human Complement C5 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60620

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Product Specifications
Precision
Recovery
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell Culture, Saliva, Milk, CSF

Sensitivity

0.1 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.

精度

Intra-Assay Precision (Precision within an assay) %CV 3.10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 9.90 (example only; lot dependent)

Recovery for Human Complement C5 ELISA Kit (Colorimetric)

Recovery

101% (example only; lot dependent)

Scientific Data Images for Human Complement C5 ELISA Kit (Colorimetric)

ELISA: Human Complement C5 ELISA Kit (Colorimetric) [NBP2-60620]

ELISA: Human Complement C5 ELISA Kit (Colorimetric) [NBP2-60620] - Representative standard curve.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Complement C5

C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190kDa glycoprotein consisting of a disulphide linked alpha-chain (115kDa) and beta-chain (75kDa). C5 occurs in plasma at about 75mg/L. It is activated via either the classical or the alternative complement pathway, by protoelytic cleavage of C5a (11.5kDa) from the N-terminal of the alpha-chain. C5a is a potent anaphylotoxin and stimulates directed migration of neutrophils, eosinophils, basophils and monocytes. The other fragment, C5b,initiates the assembly of the membrane attack complex that mediates the cytolysis of viral and bacterial pathogens.

Alternate Names

anaphylatoxin C5a analog, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, C5, complement C5, complement component 5, CPAMD4MGC142298, FLJ17816, FLJ17822

Gene Symbol

Additional Complement C5 Products

Product Documents for Human Complement C5 ELISA Kit (Colorimetric)

Download Product Datasheets

Download Manual - Human Complement C5 ELISA Kit (Colorimetric) - Sample Insert

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Human Complement C5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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