Complement Component C3a Antibody Pair [Biotin]

Novus Biologicals | Catalog # NBP3-57720

Novus Biologicals
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Key Product Details

Assay Type

Sandwich ELISA

Sample Type

Cell culture supernatants from rabbitsCan also be used to measure C3a in a wide variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as liver, spleen, kidney, and lymph nodes. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. (100 ul)

Reactivity

Rabbit
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Product Specifications

Description

Contains a capture antibody, detection antibody, and recombinant protein standard for assay development. These antibodies have been validated to function together in an ELISA format using the provided standard. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to optimize assay sensitivity and dynamic range based on their specific sample type and experimental requirements. The quantities of components are not matched, and individual components may be purchased separately. This product is for research use only.

Sample Volume Required

100 ul

Conjugate

Biotin

Scientific Data Images for Complement Component C3a Antibody Pair [Biotin]

Complement Component C3a Antibody Pair [Biotin]

ELISA: Complement Component C3a Antibody Pair [Biotin] [NBP3-57720] -

Representative standard curve for Complement Component C3a Antibody Pair [Biotin] (NBP3-57720)

Kit Contents for Complement Component C3a Antibody Pair [Biotin]

  • Capture Antibody (1 Set = <= 10 Plates; 2 Set = <= 20 Plates): (Catalog # <a href= /products/complement-component-c3a-antibody_nbp3-57627 >NBP3-57627</a>)
  • Detection Antibody (Biotinylated): (Catalog # <a href= "/products/complement-component-c3a-antibody_nbp3-57658" >NBP3-57658</a>)
  • Standard: (Catalog # <a href= "/products/complement-component-c3a-recombinant-protein_nbp3-57452" >NBP3-57452</a>)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Complement Component C3a

Complement Component C3a is an anaphylotoxin polypeptide that is proteolytically generated from C3. C3a formation is common to all three pathways of complement activation: classical (antibody-mediated), lectin and alternative. It binds C3a R on myeloid peripheral blood leukocytes, activated lymphocytes, endothelial and internal organ epithelial cells. It activates mast cells, neutrophils, and macrophages, enhances Th2 inflammatory reactions, promotes Th1 cell maturation, and inhibits Treg differentiation. It also stimulates leukocyte chemotaxis and smooth muscle contraction. Removal of the C-terminal arginine generates C3adesArg/ASP that binds the C5L2 receptor (GPR77) and stimulates triglyceride synthesis in adipocytes.

Alternate Names

Anaphylatoxin, C3

Gene Symbol

C3

Additional Complement Component C3a Products

Product Documents for Complement Component C3a Antibody Pair [Biotin]

Certificate of Analysis

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Product Specific Notices for Complement Component C3a Antibody Pair [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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