CPEB1 Antibody

Novus Biologicals | Catalog # NB100-1437

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CPEB1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PLMRNQKNRDSS corresponding to C-Terminus according to NP_085097.3, NP_001073001.1, NP_001073002.1, NP_001073003.1.

Localization

Cytoplasmic

Specificity

This antibody is expected to recognise alle reported isoforms. Variants (NP_001073002.1; NP_001073003.1) encode the same isoform.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CPEB1 Antibody

Western Blot: CPEB1 Antibody [NB100-1437]

Western Blot: CPEB1 Antibody [NB100-1437]

Western Blot: CPEB1 Antibody [NB100-1437] - Staining of human brain lysate (RIPA buffer, 35 ug total protein per lane) using this antibody at 0.5 ug/ml. Detected by western blot using chemiluminescence.

Applications for CPEB1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 65-70 kDa band observed in human brain lysates (predicted MW of 65 kDa band according to NP_085097). Please note this band was always accompanied by a slightly stronger band at approx. 48 kDa and both bands could be blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CPEB1

This gene encodes a member of the cytoplasmic polyadenylation element (CPE) binding protein family. This highly conserved protein binds to a specific RNA sequence called the CPE found in the 3' UTR of some mRNAs. Similar proteins in Xenopus and mouse function to induce cytoplasmic polyadenylation of dormant mRNAs with short polyA tails, resulting in their translation. Members of this protein family regulate translation of cyclin B1 during embryonic cell divisions. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternate Names

CPEBCEBP, CPE-binding protein 1, CPE-BP1, cytoplasmic polyadenylation element binding protein 1, cytoplasmic polyadenylation element-binding protein 1, FLJ13203, h-CEBP, hCPEB-1, MGC34136, MGC60106

Entrez Gene IDs

64506 (Human)

Gene Symbol

CPEB1

UniProt

NP_085097.3 (Human)

Additional CPEB1 Products

Product Documents for CPEB1 Antibody

Certificate of Analysis

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Product Specific Notices for CPEB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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