CpG oligodeoxynucleotides

Novus Biologicals | Catalog # NBP2-26237

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Key Product Details

Species

Mouse

Applications

Functional Assay

Product Summary for CpG oligodeoxynucleotides

Mouse Sequence CpG ODN (1585) Type A: 5' G*GGGTCAACGTTGAG*G*G*G*G*G 3'

Human/Mouse Sequence CpG ODN (2216) Type A: 5' G*GGGGACGATCGTCG*G*G*G*G*G 3'

Human Sequence CpG ODN (2006) Type B: 5' T*C*G*T*C*G*T*T*T*T*G*T*C*G*T*T*T*T*G*T*C*G*T*T 3'

Mouse Sequence CpG ODN (1826) Type B: 5' T*C*C*A*T*G*A*C*G*T*T*C*C*T*G*A*C*G*T*T 3’

Mouse Sequence CpG ODN(1668) Type B: 5' T*C*C*A*T*G*A*C*G*T*T*C*C*T*G*A*T*G*C*T 3'

Mouse Sequence CpG ODN (1018) Type B: 5' T*G*A*C*T*G*T*G*A*A*C*G*T*T*C*G*A*G*A*T*G*A 3'

Human/Mouse Sequence CpG ODN (C274) Type C: 5' T*C*G*T*C*G*A*A*C*G*T*T*C*G*A*G*A*T*G*A*T 3'

Human/Mouse Sequence CpG ODN (C695) Type C: 5' T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T 3'

Human/Mouse Sequence CpG ODN (C792) Type C: 5' T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T*C*G*A*A*T 3'

(* Indicates a phosphorothioate modification)

Functionality: This product is useful for the activation of TLR9 and the stimulation of TLR9 has been reported with 5-20 ug/ml.

Product Specifications

Applications

Ligand Activation (5 - 20 ug/ml)

Application Notes

A TLR9/NF-kB SEAP reporter construct in a HEK 293 cell line was used as a model system for studying hTLR9 activation.

Scientific Data Images for CpG oligodeoxynucleotides

CpG oligodeoxynucleotides [NBP2-26237]
CpG oligodeoxynucleotides [NBP2-26237] - Evaluation of various CpG ODN effects on activation of human TLR9. TLR9/NF-kB/SEAPorter HEK 293 cells were plated in 96-well plates at 7.5 x 104 cells/well for 16 h. Cells were treated with various CpG ODNs at different concentrations (1, 5, 10 and 20 ug/ml) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit. Note: Both negative hCpG and mCpG are the negative control ODNs.
CpG oligodeoxynucleotides [NBP2-26237] - Evaluation of various CpG ODN effects on activation of mouse TLR9. NF-kB/SEAPorte RAW cells were plated in 96-well plates at 7.5 x 10^4 cells/ well for 16 h. Cells were treated with various CpG ODNs at different concentrations (1, 5, 10 and 20 ug/ml) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit.RAW264.7 cells endogenously express functional TLR9, highly responding to CpG ODNs. Both negative hCpG and mCpG are the negative control ODNs.

Kit Contents for CpG oligodeoxynucleotides

  • NBP2-31132SS: 25 ug mouse CpG Type B ODN (1826)
  • NBP2-31134SS: 25 ug human CpG Type B ODN (2006)
  • NBP2-31136SS: 25 ug human CpG Type A ODN (2216)
  • NBP2-31137SS: 25 ug human CpG Type C ODN (C274)
  • NBP2-31138SS: 25 ug human CpG Type C ODN (C695)
  • NBP2-31139SS: 25 ug mouse CpG Type C ODN (C792)
  • NBP2-31140SS: 25 ug mouse CpG Type A ODN (1585)
  • NBP2-31141SS: 25 ug mouse CpG Type B ODN (1668)
  • NBP2-31142SS: 25 ug mouse CpG Type B ODN (1018)

Formulation, Preparation, and Storage

Formulation

Sterile water

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CpG oligodeoxynucleotides

Unmethylated deoxycytosine-deoxyguanosine (CpG) dinucleotides are more frequently present in bacterial and viral DNA than in mammalian DNA1. CpG oligodeoxynucleotides (ODNs) are synthetic forms of oligonucleotides, which contain unmethylated CpG motifs, and can induce cellular immune responses through the activation of Toll-like receptor 9 (TLR9)2,3. There are three types (types A, B and C) of CpG ODNs, each of which acts differentially on stimulation of immune cells3-5. Type A CpG ODNs consist of a phosphodiester central CpG-containing palindromic motif and a phosphorothioate poly-G string at 3'-end. Type A CpG ODNs are high inducers for IFNa production in plasmacytoid dendritic cells (pDCs) but are low inducers for TLR9-dependent NF-kB induction. Type B CpG ODNs consist of a full phosphorothioate backbone with one or more CpG motifs. They are relatively higher inducers for TLR9-dependent NF-kB induction, strongly activating B cells, but are low inducers for IFNa production in pDCs. Type C CpG ODNs contain both features of Types A and B, which consist of a full phosphorothioate backbone and a CpG-containing palindromic motif. Type C CpG ODNs can induce high IFNa production in pDCs as well as B cell activation.

Alternate Names

CpG ODN, CpG ODN (2006), oligodeoxynucleotides (ODN)

Additional CpG oligodeoxynucleotides Products

Product Documents for CpG oligodeoxynucleotides

Certificate of Analysis

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Product Specific Notices for CpG oligodeoxynucleotides

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Citations for CpG oligodeoxynucleotides

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Protocols

View specific protocols for CpG oligodeoxynucleotides (NBP2-26237):

CpG oligodeoxynucleotides:
Hazard Information
Chemical Name CPG ODN 2006 & Negative Control
Chemical Formula CpG ODN- 5' T*C*G*T*C*G*T*T*T*T*G*T*C*G*T*T*T*T*G*T*C*G*T*T 3' (* Phosphorothioate bonds)
CAS Number N/A
EEC-No N/A

First Aid Measures
Eye Contact May cause eye irritation.
Skin Contact May cause skin irritation. May be harmful if absorbed through the skin.
Inhalation May causes respiratory tract irritation. May be harmful if inhaled.
Ingestion May be harmful if swallowed.

Fire Fighting Measures
Special risks: N/A
Suitable Extinguish Media: Water spray, carbon dioxide, dry chemical powder or appropriate foam.

Accidental Release Measures
Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Consult a physician.
Skin: Get medical aid immediately. Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. SPEEDY ACTION IS CRITICAL!
Ingestion: If swallowed, get medical aid immediately. Only induce vomiting if directed to do so by medical personnel. Never give anything by mouth to an unconscious person.
Inhalation: Remove from exposure and move to fresh air immediately. If breathing is difficult, give oxygen. SPEED IS ESSENTIAL, OBTAIN MEDICAL AID IMMEDIATELY. Do not use mouth-to-mouth resuscitation if victim ingested or inhaled the substance; induce artificial respiration with the aid of a pocket mask equipped with a one-way valve or other proper respiratory medical device.
Notes: Consult a physician. Show this safety data sheet to the physician. Move away from the dangerous area.

Handling and Storage
Handling Avoid contact with eyes, skin and clothing. Avoid prolonged or repeated exposure. Avoid inhalation. Use personal protective equipment (i.e. impermeable gloves, lab coat or apron).
Store at -20 degrees C. Store in a tightly closed container. Stable under recommended storage conditions

Exposure Controls / Personal Protection
Physical and Chemical Properties
Form Powder
Color Colorless
Odor Odorless
Melting Point No data available
Boiling Temperature No data available
Density No data available
Vapor Pressure No data available
Solubility in Water Very soluble
Flash Point No data available
Explosion limits No data available
Ignition Temperature No data available

Stability and Reactivity
Stability: Stable
Hazardous Polymerization: Will not occur.
Materials to avoid: Strong oxidizing agents.
Hazardous Decomposition Products: Decomposition products are not hazardous.
HMIS Classification: Health Hazard 0, Flammability Hazard 0, Reactivity Hazard 0
NFPA Rating: Health Hazard 0, Fire 0, Reactivity Hazard 0.

Disposal Considerations
Observe all federal, state, and local environmental regulations. Contact a licensed professional waste disposal service to dispose of this material. Must not be disposed together with household garbage.

Other Information
The information contained in this material safety datasheet is believed to be accurate but it is the responsibility of the user or supplier to determine the applicability of these data to the formulation of necessary safety precautions.
NOVUS shall not be held responsible for any damage resulting from the use of the above product or the information contained in this material safety data sheet.

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