Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-60776

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Guinea Pig (100%), Hamster (100%), Primate (100%), Rabbit (100%), Rat (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Creatine kinase MT 1A Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr153 conjugated to KLH. Accession # P12532

Reactivity Notes

This antibody detects both Isoform 1 and 2 of the protein.

Modification

p Tyr153

Specificity

Specific for endogenous levels of the ~46 kDa Creatine kinase MT 1A protein phosphorylated at Tyr153. Immunolabeling of Creatine kinase MT 1A is greatly decreased by lambda-phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free

Western Blot: Creatine kinase MT 1A [p Tyr153] Antibody [NBP2-60776]

Western Blot: Creatine kinase MT 1A [p Tyr153] Antibody [NBP2-60776]

Western Blot: Creatine kinase MT 1A [p Tyr153] Antibody [NBP2-60776] - Western blot of mouse mitochondria from whole brain lysateshowing specific immunolabeling of the ~46 kDa CK1Mt proteinphosphorylated at Tyr153 in the first lane (-). Phosphospecificityis shown in the second lane (+) where the immunolabeling isgreatly decreased by blot treatment with lambda phosphatase(Lambda-Ptase, 1200 units for 30 minutes).

Applications for Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Creatine kinase MT 1A

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to thecytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes,sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two differentoligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes.Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; thismay be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrialcreatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes locatednear each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.(provided by RefSeq)

Alternate Names

Acidic-Type Mitochondrial Creatine Kinase, CKMT, CKMT1, CKMT1A, Creatine Kinase U-Type, Mitochondrial, Creatine Kinase, Mitochondrial 1 (Ubiquitous), Creatine Kinase, Mitochondrial 1A, EC 2.7.3.2, Mia-CK, Ubiquitous Mitochondrial Creatine Kinase, U-MtCK

Gene Symbol

CKMT1A

Additional Creatine kinase MT 1A Products

Product Documents for Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for Creatine kinase MT 1A [p Tyr153] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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