CrkRS Antibody - BSA Free

Novus Biologicals | Catalog # NB100-87011

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CrkRS Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human cell division cycle 2 (CDC2)-related kinase arginine/serine-rich using the numbering given in entry NP_057591.2 (GeneID 51755).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CrkRS Antibody - BSA Free

Western Blot: CrkRS Antibody [NB100-87011]

Western Blot: CrkRS Antibody [NB100-87011]

Western Blot: CrkRS Antibody [NB100-87011] - Detection of Human and Mouse CrkRS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: CrkRS Antibody [NB100-87011]

Immunoprecipitation: CrkRS Antibody [NB100-87011]

Immunoprecipitation: CrkRS Antibody [NB100-87011] - Detection of human CrkRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for IP at 6 ug per reaction. For blotting immunoprecipitated CrkRS, NB100-87011 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for CrkRS Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in WB reported in scientific literature ( PMID 24478330).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CrkRS

CrkRS (Cdc2-related kinase, arginine/serine-rich) is a Cdc-2 related kinase that contains an arginine- and serine-rich (SR) domain and has recently been reported to interact with cyclin L1 and L2. Since it has been discovered that CrkRs pairs with a cyclin partner, it has also been given the name CDK12. CrkRs localizes to nuclear speckles and immunoprecipitates with RNA polymerse II and the SF2/ASF splicing factor and is proposed to play a role in the regulation of alternative splicing. Alternate names for CrkRS include cell division cycle 2-related protein kinase 7, CDC2-related protein kinase 7, CRKRS, CRK7, and KIAA0904.

Alternate Names

Cdc2-related kinase, arginine/serine-richCrkRS, Cell division cycle 2-related protein kinase 7, Cell division protein kinase 12, CRK7CDC2-related protein kinase 7, CRKR, CRKRS, cyclin-dependent kinase 12, EC 2.7.11, EC 2.7.11.23, hCDK12, KIAA0904EC 2.7.11.22

Entrez Gene IDs

51755 (Human)

Gene Symbol

CDK12

UniProt

Q9NYV4 (Human)

Additional CrkRS Products

Product Documents for CrkRS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CrkRS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CrkRS Antibody - BSA Free

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