CRLF1 Antibody (4F4) - Azide and BSA Free

Novus Biologicals | Catalog # H00009244-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4F4

Format

Azide and BSA Free
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Product Specifications

Immunogen

CRLF1 (NP_004741, 135 a.a. ~ 230 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PEKPVNISCWSKNMKDLTCRWTPGAHGETFLHTNYSLKYKLRWYGQDNTCEEYHTVGPHSCHIPKDLALFTPYEIWVEATNRLGSARSDVLTLDIL

Specificity

CRLF1 - cytokine receptor-like factor 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CRLF1 Antibody (4F4) - Azide and BSA Free

Western Blot: CRLF1 Antibody (4F4) [H00009244-M01]

Western Blot: CRLF1 Antibody (4F4) [H00009244-M01]

Western Blot: CRLF1 Antibody (4F4) [H00009244-M01] - CRLF1 monoclonal antibody (M01), clone 4F4 Analysis of CRLF1 expression in HeLa.
Western Blot: CRLF1 Antibody (4F4) [H00009244-M01]

Western Blot: CRLF1 Antibody (4F4) [H00009244-M01]

Western Blot: CRLF1 Antibody (4F4) [H00009244-M01] - Analysis of CRLF1 over-expressed 293 cell line, cotransfected with CRLF1 Validated Chimera RNAi ( Cat # H00009244-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CRLF1 monoclonal antibody (M01), clone 4F4 (Cat # H00009244-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: CRLF1 Antibody (4F4) [H00009244-M01]

Western Blot: CRLF1 Antibody (4F4) [H00009244-M01]

Western Blot: CRLF1 Antibody (4F4) [H00009244-M01] - Analysis of CRLF1 expression in transfected 293T cell line by CRLF1 monoclonal antibody (M01), clone 4F4.Lane 1: CRLF1 transfected lysate(46.3 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: CRLF1 Antibody (4F4) [H00009244-M01]

Immunoprecipitation: CRLF1 Antibody (4F4) [H00009244-M01]

Immunoprecipitation: CRLF1 Antibody (4F4) [H00009244-M01] - Analysis of CRLF1 transfected lysate using anti-CRLF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CRLF1 MaxPab rabbit polyclonal antibody.
ELISA: CRLF1 Antibody (4F4) [H00009244-M01]

ELISA: CRLF1 Antibody (4F4) [H00009244-M01]

ELISA: CRLF1 Antibody (4F4) [H00009244-M01] - Detection limit for recombinant GST tagged CRLF1 is approximately 0.03ng/ml as a capture antibody.

Applications for CRLF1 Antibody (4F4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CLF-1

The CRLF1 gene encodes a member of the cytokine type I receptor family. The protein forms a secreted complex with cardiotrophin-like cytokine factor 1 and acts on cells expressing ciliary neurotrophic factor receptors. The complex can promote survival of neuro

Long Name

Cytokine-like Factor 1

Alternate Names

CLF1, CRLF-1, CRLF1

Entrez Gene IDs

9244 (Human)

Gene Symbol

CRLF1

OMIM

272430 (Human)

UniProt

NP_004741

Additional CLF-1 Products

Product Documents for CRLF1 Antibody (4F4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CRLF1 Antibody (4F4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

IL-6 Family

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