DC-SIGN/CD209 Antibody (JE31-24)

Name: DC-SIGN/CD209 Antibody (JE31-24)
Brand: Novus Biologicals
SKU: NBP3-32258
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-32258

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE31-24 expressed in HEK293

View all DC-SIGN/CD209 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human DC-SIGN/CD209 aa 1-100 / 404. (Uniprot: Q9NNX6)

Localization

Cell membrane; Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DC-SIGN/CD209 Antibody (JE31-24)

Western Blot: DC-SIGN/CD209 Antibody (JE31-24) [NBP3-32258]

Western Blot: DC-SIGN/CD209 Antibody (JE31-24) [NBP3-32258] - Western blot analysis of DC-SIGN/CD209 on different lysates with Rabbit anti-DC-SIGN/CD209 antibody (NBP3-32258) at 1/1,000 dilution.

Lane 1: A549 cell lysate (15 ug/Lane)
Lane 2: Human skin tissue lysate (30 ug/Lane)
Lane 3: Human placenta tissue lysate (30 ug/Lane)

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32258) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for DC-SIGN/CD209 Antibody (JE31-24)

Application

Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DC-SIGN/CD209

DC-SIGN encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1

Alternate Names

CD209, CLEC4L, DC-SIGN1, DCSIGN

Gene Symbol

CD209

Additional DC-SIGN/CD209 Products

Product Documents for DC-SIGN/CD209 Antibody (JE31-24)

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Product Specific Notices for DC-SIGN/CD209 Antibody (JE31-24)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.