DHX8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35462

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DHX8 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1001-1220 of human DHX8 (NP_004932.1).

Sequence:
CSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DHX8 Antibody - BSA Free

DHX8 Antibody

Western Blot: DHX8 Antibody [NBP3-35462] -

Western Blot: DHX8 Antibody [NBP3-35462] - Western blot analysis of various lysates using DHX8 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for DHX8 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DHX8

DHX8 is a member of the DEAD box protein family, which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). The DEAD or DEAH motif is characteristic of a family of putative ATP-dependent RNA helicases. DHX8 has a role in nuclear export of spliced mRNA by releasing the RNA from the spliceosome.

Alternate Names

DDX8ATP-dependent RNA helicase DHX8, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase), DEAH (Asp-Glu-Ala-His) box polypeptide 8, DEAH box protein 8, DEAH-box protein 8, EC 3.6.1, EC 3.6.4.13, HRH1RNA helicase HRH1, PRP22, PRPF22

Gene Symbol

DHX8

Additional DHX8 Products

Product Documents for DHX8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DHX8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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