DLG5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-28739

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DLG5 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1325 and 1375 of human discs, large homolog 5 using the numbering given in entry NP_004738.3 (GeneID 9231).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DLG5 Antibody - BSA Free

Western Blot: DLG5 Antibody [NBP1-28739]

Western Blot: DLG5 Antibody [NBP1-28739]

Western Blot: DLG5 Antibody [NBP1-28739] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-DLG5 antibody NBP1-28739 used for WB at 0.1 ug/mL (A) and 1 ug/mL (B) and used for IP at 10 ug/mg lysate. DLG5 was also immunoprecipitated by rabbit anti-DLG5 antibody NBP1-28694, which recognizes an upstream epitope. Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Immunoprecipitation: DLG5 Antibody [NBP1-28739]

Immunoprecipitation: DLG5 Antibody [NBP1-28739]

Immunoprecipitation: DLG5 Antibody [NBP1-28739] - IP of overexpressed CitK (citron-kinase) in HEK293 cell line. DLG5 is co-immunoprecipitated with the DLG5 antibody but not with control beads or with a control isotypic antibody. Image submitted by a verified customer review.

Applications for DLG5 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
DLG5 antibody validated for IP from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NBP1-28739 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DLG5

DLG5 (discs large homolog 5) is a member of the family of discs large (DLG) homologs, a subset of the membrane-associated guanylate kinase (MAGUK) superfamily. The MAGUK proteins are composed of a catalytically inactive guanylate kinase domain, in addition to PDZ and SH3 domains, and are thought to function as scaffolding molecules at sites of cell-cell contact. DLG5 localizes to the plasma membrane and cytoplasm, and interacts with components of adherens junctions and the cytoskeleton. It is proposed to function in the transmission of extracellular signals to the cytoskeleton and in the maintenance of epithelial cell structure. [from NCBI GeneID:9231]

Alternate Names

discs, large homolog 5 (Drosophila), KIAA0583large (Drosophila) homolog 5, LP-DLG

Entrez Gene IDs

9231 (Human)

Gene Symbol

DLG5

UniProt

NP_004738.3 (Human)

Additional DLG5 Products

Product Documents for DLG5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DLG5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DLG5 Antibody - BSA Free (1)

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  • DLG5 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: HEK 293 cell line and 293T cell lysate
    Species: Human
    Verified Customer | Posted 07/10/2019
    Immunoprecipitation of overexpress CitK (citron-kinase) in HEK293 cell line. DLG5 is co-immunoprecipitated with r alpha DLG5 antibody (Novus NBP1 28739) but not with controls beads alone or with control isotypic antibody.
    DLG5 Antibody - BSA Free NBP1-28739

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