DNA Polymerase lambda Antibody

Novus Biologicals | Catalog # NB100-1358

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DNA Polymerase lambda Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-LGLPYREPAERDW corresponding to C-Terminus according to NP_037406.1.

Localization

Nuclear

Specificity

This product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core), but considering only the peptide that we used as immunogen, the two share the sequence YREP**R** at the C-terminus. Furthermore, the protein structures appear to be very similar in this region.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DNA Polymerase lambda Antibody

Western Blot: DNA Polymerase lambda Antibody [NB100-1358]

Western Blot: DNA Polymerase lambda Antibody [NB100-1358]

Western Blot: DNA Polymerase lambda Antibody [NB100-1358] - Staining of A549 (A) and HepG2 (B) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for DNA Polymerase lambda Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5-1 ug/ml
Application Notes
WB: Approx 60 kDa band observed in cell lysates of A549 and approx 60-70 kDa in cell lysates of HepG2 (calculated MW of 63.5k Da according to Human NP_037406.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNA Polymerase lambda

GenBank Accession Number - NP_037406 DNA polymerase lambda (Pol lambda) is a member of the Pol X family that has properties in common with several other DNA polymerases. The specific role of Pol lambda has been speculated to be in non-homologous end-joining including DNA strand-breaks resulting from DNA damage.

Alternate Names

BETAN, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase kappa, DNA polymerase lambda, EC 2.7.7.7, EC 4.2.99.-, FLJ46002, Pol beta2, Pol Lambda, POLKAPPA, polymerase (DNA directed), lambda

Entrez Gene IDs

27343 (Human); 56626 (Mouse)

Gene Symbol

POLL

UniProt

NP_037406. (Human)

Additional DNA Polymerase lambda Products

Product Documents for DNA Polymerase lambda Antibody

Certificate of Analysis

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Product Specific Notices for DNA Polymerase lambda Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DNA Polymerase lambda Antibody

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