DNA Polymerase lambda Antibody

Novus Biologicals | Catalog # NB100-81664

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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DNA Polymerase lambda Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human polymerase (DNA directed), lambda using the numbering given in entry NP_037406.1 (GeneID 27343).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNA Polymerase lambda Antibody

Western Blot: DNA Polymerase lambda Antibody [NB100-81664]

Western Blot: DNA Polymerase lambda Antibody [NB100-81664]

Western Blot: DNA Polymerase lambda Antibody [NB100-81664] - Detection of Human Pol Lambda by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody NB100-81664 used for WB at 0.1 ug/ml. Detection : Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81664]

Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81664]

Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81664] - Detection of human Polymerase lambda by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody NB100-81664 used for IP at 6 ug per reaction. Pol Lambda was also immunoprecipitated by rabbit anti-Pol Lambda antibody NB100-81665. For blotting immunoprecipitated Pol Lambda, NB100-81664 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for DNA Polymerase lambda Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DNA Polymerase lambda

DNA polymerase lambda (pol lambda) is a DNA polymerase involved in base excision repair (BER). Pol lambda possesses terminal transferase and 5'-deoxyribose-5-phosphate lyase activity. These activities have been found to be coordinated by PCNA and replication protein A. Alternate names for pol lambda include DNA polymerase kappa, DNA polymerase beta-2, pol beta-2, POLL, POLL-KAPPA, and BETA-N.

Alternate Names

BETAN, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase kappa, DNA polymerase lambda, EC 2.7.7.7, EC 4.2.99.-, FLJ46002, Pol beta2, Pol Lambda, POLKAPPA, polymerase (DNA directed), lambda

Entrez Gene IDs

27343 (Human)

Gene Symbol

POLL

UniProt

Q9UGP5 (Human)

Additional DNA Polymerase lambda Products

Product Documents for DNA Polymerase lambda Antibody

Certificate of Analysis

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Product Specific Notices for DNA Polymerase lambda Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DNA Polymerase lambda Antibody

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