DNA polymerase sigma Antibody (2F8) - Azide and BSA Free

Novus Biologicals | Catalog # H00011044-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2F8

Format

Azide and BSA Free
View all DNA polymerase sigma Primary Antibodies »

Product Specifications

Immunogen

POLS (NP_008930, 2 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SPCPEEAAMRREVVKRIETVVKDLWPTADVQIFGSFSTGLYLPTSDIDLVVFGKWERPPLQLLEQALRKHNVAEPCSIKVLDKATVPIIKLTDQETEVKVDISFNMETG

Specificity

POLS - polymerase (DNA directed) sigma

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for DNA polymerase sigma Antibody (2F8) - Azide and BSA Free

Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01]

Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01]

Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01] - Analysis of POLS over-expressed 293 cell line, cotransfected with POLS Validated Chimera RNAi ( Cat # H00011044-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POLS monoclonal antibody (M01), clone 2F8 (Cat # H00011044-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01]

Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01]

Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01] - Analysis of POLS expression in transfected 293T cell line by POLS monoclonal antibody (M01), clone 2F8. Lane 1: POLS transfected lysate (59.9 KDa). Lane 2: Non-transfected lysate.
ELISA: DNA polymerase sigma Antibody (2F8) [H00011044-M01]

ELISA: DNA polymerase sigma Antibody (2F8) [H00011044-M01]

ELISA: DNA polymerase sigma Antibody (2F8) [H00011044-M01] - Detection limit for recombinant GST tagged POLS is approximately 0.3ng/ml as a capture antibody.

Applications for DNA polymerase sigma Antibody (2F8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DNA polymerase sigma

The protein encoded by this gene is a DNA polymerase that is likely involved in DNA repair. In addition, the encoded protein may be required for sister chromatid adhesion.

Alternate Names

DNA polymerase kappa, DNA polymerase sigma, EC 2.7.7.7, LAK-1TUTase 5, PAP associated domain containing 7, POLKTRF41, POLSTUTASE5, polymerase (DNA directed) sigma, polymerase (DNA-directed) sigma, Terminal uridylyltransferase 5, Topoisomerase-related function protein 4-1, TRF4-1LAK1, TRF4PAP-associated domain-containing protein 7

Entrez Gene IDs

11044 (Human)

Gene Symbol

TENT4A

OMIM

605198 (Human)

UniProt

NP_008930 (Human)

Additional DNA polymerase sigma Products

Product Documents for DNA polymerase sigma Antibody (2F8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DNA polymerase sigma Antibody (2F8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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