DREF Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76327

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DREF Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide from near the center of human ZBED1. The immunogen is located within amino acids 180 - 230 of ZBED1. Amino Acid Squence: STDMWRSENQNRAYV

Specificity

This antibody is predicted to have no cross-reactivity to ZBED2 or ZBED3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DREF Antibody - BSA Free

Western Blot: DREF AntibodyBSA Free [NBP1-76327]

Western Blot: DREF AntibodyBSA Free [NBP1-76327]

Western Blot: DREF Antibody [NBP1-76327] - A549 cell lysate with DREF antibody at 1 ug/ml.

Applications for DREF Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DREF

ZBED1, also known as DREF, is the human homolog of the Drosophila protein DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation. RNA interference (RNAi) experiments targeting ZBED1 RNA resulted in the inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells, suggesting that ZBED1 also plays a role in cell proliferation in human cells. At least 22 ribosomal proteins (RPs) possess ZBED1 binding sites in their promoters; anti-ZBED1 RNAi also resulted in decreased RP gene expression. Immunoprecipitation analyses have shown that ZBED1 self-associates in vivo via a carboxyl-terminal hATC domain, and this self-association is required for nuclear localization and DNA binding. This antibody is predicted to have no cross-reactivity to ZBED2 or ZBED3.

Alternate Names

Ac-like transposable element, ALTEDNA replication-related element binding factor, hDREF, KIAA0785dREF homolog, Putative Ac-like transposable element, putative Ac-like transposon, TRAMPDREFBED-type zinc finger domain-containing protein 1, zinc finger BED domain-containing protein 1, zinc finger, BED-type containing 1

Gene Symbol

ZBED1

UniProt

AAH15030 (Human)

Additional DREF Products

Product Documents for DREF Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DREF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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