Multi-Species dsRNA ELISA Kit (Colorimetric)

3 ng/ml

1 - 300 ng/ml (0.1 - 30 ng/well)

Sandwich ELISA

ELISA Kit (Colorimetric)

Multi-Species

We recommend using the kit to detect viral dsRNAs or large natural or synthetic dsRNAs of non-viral origin in nucleic acid extracts, as well as to detect the presence of undesired dsRNA molecules in artificially synthesized (m)RNA preparations. Serial dilutions of the Poly(I:C) dsRNA standard (included in the kit) can be used as a positive control. For the exact detection protocol we refer to the kit manual that can be downloaded from our website

Caution: The Poly(I:C) dsRNA positive control included in this sandwich ELISA kit is not intended to be used as a quantitative standard for other dsRNA preparations. Low molecular weight polyinosine-polycytidylic acid (i.e. poly(I:C)) is a synthetic analog of double-stranded RNA (dsRNA) and is comprised of short strands of inosine poly(I) homopolymer annealed to strands of cytidine poly(C) homopolymer. The average size of our Poly(I:C) LMW is 0.2 kb to 1 kb. The anti dsRNA antibodies J2 and/or K2 used in this kit may exhibit a different degree of reactivity with different dsRNAs obtained from synthetic or natural sources. It is therefore only intended to be used as a positive control to see if the ELISA has been executed correctly and that the test shows a linear relationship between the amount of dsRNA and the read out for the OD450.

This assay works on the sandwich-ELISA principle and uses the K1 (IgG2a) mouse monoclonal antibody to dsRNA as a catcher antibody. The monoclonal antibody K2 (IgM) is used as the detector antibody. Based on the use of two double-stranded RNA (dsRNA)-specific monoclonal antibodies the dsRNA Detection Kit allows sensitive and selective detection of dsRNA molecules (larger than 30-40 bp), independent of their nucleotide composition and sequence. The detection is highly specific: dsRNA can be detected in nucleic acid extracts in the presence of 1,000-10,000-fold excess of other nucleic acids.

Source
The two dsRNA antibodies used in this kit were produced as follows: Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clones K1 and K2.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.