dTAG-13

Tocris Bioscience | Catalog # 6605

Degrades mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation
Tocris Bioscience

Key Product Details

Description

Degrades mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation
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Product Description

dTAG-13 is a degrader targeting mutant FKBP12F36V fusion proteins. Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a cereblon-binding ligand. Application of dTAG-13 induces rapid, reversible and selective degradation of FKBP12F36V fusion proteins in vitro and in vivo. dTAG is generalizable to a range of fusion proteins; useful as an alternative to genetic methods for target validation.

Negative control (Cat. No. 6916) also available.

FKBP12F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques, via transgene expression or CRISPR-mediated locus-specific knock-in.

Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12F36V are available from Addgene.

Licensing Information

Sold under license from Dana-Farber Cancer Institute

Product Specifications for dTAG-13

Molecular Weight

1049.18

Formula

C57H68N4O15

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

1-[(2S)-1-Oxo-2-(3,4,5-trimethoxyphenyl)butyl]-(2S)-2-piperidinecarboxylate (1R)-3-(3,4-dimethoxyphenyl)-1-[2-[2-[[6-[[2-(2,6-dioxo-3-piperidinyl)-2,3-dihydro-1,3-dioxo-1H-isoindol-4-yl]oxy]hexyl]amino]-2-oxoethoxy]phenyl]propyl ester

CAS Number

2064175-41-1

PubChem ID

124187630

InChI Key

BJFBRLAWLPZOMJ-QHVFGHLPSA-N

SMILES

CC[C@@H](C1=CC(OC)=C(C(OC)=C1)OC)C(N2CCCC[C@H]2C(O[C@@H](C3=C(C=CC=C3)OCC(NCCCCCCOC4=C5C(N(C(C5=CC=C4)=O)C6CCC(NC6=O)=O)=O)=O)CCC7=CC(OC)=C(C=C7)OC)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 52.46 50
Ethanol 20.98 20

Preparing Stock Solutions for dTAG-13

The following data is based on the product molecular weight 1049.18.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 1.91 mL 9.53 mL 19.06 mL
2.5 mM 0.38 mL 1.91 mL 3.81 mL
5 mM 0.19 mL 0.95 mL 1.91 mL
25 mM 0.04 mL 0.19 mL 0.38 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 121 publications citing the usage of this product.

Product Documents for dTAG-13

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for dTAG-13

For research use only

Customer Reviews for dTAG-13 (4)

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  • Degradation of Myc fusion protein
    Name: Bikesh Nirala
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Murine cell lines
    Verified Customer | Posted 06/21/2022
    I tested this product to degrade FFKBP12-Myc protein. It is working nicely.
    dTAG-13 6605
  • dTAG-13 treatment of FKBP tagged over expressed protein in glioma cell lines
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Glioma
    Verified Customer | Posted 07/02/2020
    Assayed the degradation of FKBP-tagged-protein with HA tag in glioma cells using dTAG-13 at 500nM concentration for 0h, 4h, 6h and 24h time intervals. Untransfected cells has no HA signal and in transfected cells with dTAG-13 treatment lead to complete degradation of FKBP-tagged-protein in whole cell lysates
    dTAG-13 6605
  • Knockdown of FKBP-tagged Chromatin Protein
    Name: Sam Weeks
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: mESC
    Verified Customer | Posted 01/30/2020
    Assayed different dTag concentrations (50nm, 250nm, 500nm) for the knockdown of a FKBP-tagged chromatin-bound protein (180kDa). Whole cell lysate (RIPA) revealed no change to WT cells (150kDa) and considerable knockdown in all conditions after just an hour of treatment.
    dTAG-13 6605
  • Works better than expected
    Name: Abderhman Abuhashem
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: E14 (mouse Embryonic Stem cells)
    Verified Customer | Posted 10/14/2019
    This is a western blot probed for HA tag to track degradation. All samples are treated with 500nM dTAG-13. Lanes are: 1) parental cell line, 2) control (no treatment), 3) 30 mins of treatment, 4) 1hr of treatment, 5) 2hrs of treatment, 6) 4hrs of treatment, 7) 6hrs of treatment. The constructs, as shown by the western, was degraded almost entirely (>98% quantified), within 30 mins. I have tested batch 2 of this product at conc. as low as 50nM and still just as effective. No apparent toxicity to cells. Extremely satisfied.
    Used dTAG-13 to degrade a transgene tagged with FKBM12-F36V and HA tags to visualize the protein.
    dTAG-13 6605

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