dTAG-7

Tocris Bioscience | Catalog # 6912

First generation Degrader for mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation
Tocris Bioscience

Key Product Details

Description

First generation Degrader for mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation
View all TAG Degradation Platforms »

Product Description

dTAG-7 is a first generation Degrader for mutant FKBP12F36V fusion proteins. Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a cereblon-binding ligand. Application of dTAG-7 induces rapid, reversible and selective degradation of FKBP12F36V fusion proteins in vitro and in vivo. dTAG is generalizable to a range of fusion proteins; useful as an alternative to genetic methods for target validation. See also dTAG-13.

FKBP12F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques, via transgene expression or CRISPR-mediated locus-specific knock-in.

Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12F36V are available from Addgene.

Licensing Information

Sold under license from Dana-Farber Cancer Institute

Product Specifications for dTAG-7

Molecular Weight

1210.32

Formula

C63H79N5O19

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(1R)-3-(3,4-Dimethoxyphenyl)-1-(2-((19-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)-2,18-dioxo-7,10,13-trioxa-3,17-diazanonadecyl)oxy)phenyl)propyl (2S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate

CAS Number

2064175-32-0

PubChem ID

131954816

InChI Key

IFCAWDLUIZXIPI-FJDAOBEISA-N

SMILES

CC[C@H](C(=O)N1CCCC[C@H]1C(=O)O[C@H](CCC1=CC(OC)=C(OC)C=C1)C1=C(OCC(=O)NCCCOCCOCCOCCCNC(=O)COC2=C3C(=O)N(C4CCC(=O)NC4=O)C(=O)C3=CC=C2)C=CC=C1)C1=CC(OC)=C(OC)C(OC)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 121.03 100
Ethanol 24.21 20

Preparing Stock Solutions for dTAG-7

The following data is based on the product molecular weight 1210.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 0.83 mL 4.13 mL 8.26 mL
5 mM 0.17 mL 0.83 mL 1.65 mL
10 mM 0.08 mL 0.41 mL 0.83 mL
50 mM 0.02 mL 0.08 mL 0.17 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 10 publications citing the usage of this product.

Product Documents for dTAG-7

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for dTAG-7

For research use only

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