EBSS, Ca and Mg salts, phenol red

Earle’s Balanced Salt Solution (EBSS) is an isotonic buffer solution containing inorganic salts and a carbohydrate as an energy source. It is used for various cell culture applications including the short-term maintenance of cells in a CO₂ environment, washing cells before dissociation or counting, and transporting cells or tissues. EBSS is manufactured with and without calcium and magnesium salts, and with and without phenol red.