A Guide to the Colony Forming Cell Assay: Methods and Tips

This protocol is intended as a guide only.

This video protocol provides step-by-step instructions on how to consistently perform the Colony Forming Cell (CFC) Assay. Tips are provided throughout the video to help optimize the assay procedure, including tricks to accurately evaluate and score colony formations.

  • 00:10 CFC Assay Overview
  • 00:54 Aliquoting the Media
  • 02:06 Preparing the Cells
  • 04:05 Plating the Cells
  • 05:14 Counting the Cells
  • 05:53 Tips for Colony Identification

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