Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EIF3C Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide derived from a sequence between residues 850 and 918 (the C-terminus) of human eukaryotic translation initiation factor 3, subunit 8, 110kDa using the numbering given in Swiss-Prot entry Q99613 (GeneID 8663).

Reactivity Notes

Mouse reactivity reported in (PMID: 28241004).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EIF3C Antibody - BSA Free

Western Blot: EIF3C Antibody [NB100-511]

Western Blot: EIF3C Antibody [NB100-511]

Western Blot: EIF3C Antibody [NB100-511] - eIF3C expression in naive and activated (overnight) murine B cells. Image submitted by a verified customer review.
Western Blot: EIF3C Antibody [NB100-511]

Western Blot: EIF3C Antibody [NB100-511]

Western Blot: EIF3C Antibody [NB100-511] - Detection of Human eIF3C/eIF3S8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3C/eIF3S8 antibody NB100-511 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: EIF3C Antibody [NB100-511]

Immunoprecipitation: EIF3C Antibody [NB100-511]

Immunoprecipitation: EIF3C Antibody [NB100-511] - Detection of human eIF3C/eIF3S8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3C/eIF3S8 antibody NB100-511 used for IP at 6 ug per reaction. eIF3C/eIF3S8 was inefficiently immunoprecipitated by rabbit anti-eIF3C/eIF3S8 antibody from Company B. For blotting immunoprecipitated eIF3C/eIF3S8, NB100-511 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
EIF3C Antibody - BSA Free

Western Blot: EIF3C Antibody - BSA Free [NB100-511] -

EIF3C is a potential target for CFH to play a role in inhibiting FLSs and monocyte function. Monocytes and FLS from RA patients were selected respectively, and cells from each patient were divided into two groups. One group was left untreated, and one group was treated with 5 μg/ml CFH for 6 h. A Volcano plot indicated up-regulated (red dots) and down-regulated (blue dots) genes (P value < 0.05 and |log2FC|> 0.5) by RNA sequencing in CFH-treated versus untreated RA monocytes. EIF3C is indicated. B Volcano plot indicated up-regulated (red dots) and down-regulated (blue dots) genes (P value < 0.05 and |log2FC|> 0.5) by RNA sequencing in CFH-treated versus untreated RA monocytes. EIF3C and EIF3CL are indicated. C We selected the genes upregulated in the CFH-treated monocytes and the genes upregulated in the CFH-treated FLS identified by RNA-sequencing and determined the intersecting proteins. We obtained one gene, namely EIF3C. DEGs: Differentially Expressed Genes. D The boxplot shows the relative expression of EIF3C and EIF3CL. E CFH upregulated the protein expression of EIF3C by using Western blot. F CFH combined with TNF-alpha upregulated protein expression of EIF3C in both RA FLSs and monocytes by using Western blot. Data are expressed as mean +/- SEM (n = 6). *p < 0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37996918), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
EIF3C Antibody - BSA Free

Western Blot: EIF3C Antibody - BSA Free [NB100-511] -

EIF3C is a potential target for CFH to play a role in inhibiting FLSs and monocyte function. Monocytes and FLS from RA patients were selected respectively, and cells from each patient were divided into two groups. One group was left untreated, and one group was treated with 5 μg/ml CFH for 6 h. A Volcano plot indicated up-regulated (red dots) and down-regulated (blue dots) genes (P value < 0.05 and |log2FC|> 0.5) by RNA sequencing in CFH-treated versus untreated RA monocytes. EIF3C is indicated. B Volcano plot indicated up-regulated (red dots) and down-regulated (blue dots) genes (P value < 0.05 and |log2FC|> 0.5) by RNA sequencing in CFH-treated versus untreated RA monocytes. EIF3C and EIF3CL are indicated. C We selected the genes upregulated in the CFH-treated monocytes and the genes upregulated in the CFH-treated FLS identified by RNA-sequencing and determined the intersecting proteins. We obtained one gene, namely EIF3C. DEGs: Differentially Expressed Genes. D The boxplot shows the relative expression of EIF3C and EIF3CL. E CFH upregulated the protein expression of EIF3C by using Western blot. F CFH combined with TNF-alpha upregulated protein expression of EIF3C in both RA FLSs and monocytes by using Western blot. Data are expressed as mean +/- SEM (n = 6). *p < 0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37996918), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for EIF3C Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
EIF3C antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-511 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EIF3C

eIF3S8 (eukaryotic translation initiation factor 3 subunit 8) is one of 13 non-identical subunits that comprise the eIF-3 (eukaryotic translation initiation factor-3) complex. eIF-3 plays a role in ribosome dissociation, the stimulation of mRNA binding, and the stabilization of the ternary complex for the initiation of protein synthesis.

Alternate Names

cell migration-inducing protein 17, eIF3 p110, eIF3c, EIF3CL, eIF3-p110, EIF3S8FLJ55450, Eukaryotic translation initiation factor 3 subunit 8, eukaryotic translation initiation factor 3 subunit C, eukaryotic translation initiation factor 3, subunit 8 (110kD), eukaryotic translation initiation factor 3, subunit 8, 110kDa, eukaryotic translation initiation factor 3, subunit C, FLJ53378, FLJ54400, FLJ54404, FLJ55750, FLJ78287, MGC189737, MGC189744

Entrez Gene IDs

8663 (Human); 56347 (Mouse); 293484 (Rat)

Gene Symbol

EIF3C

UniProt

Q99613 (Human)

Additional EIF3C Products

Product Documents for EIF3C Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EIF3C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EIF3C Antibody - BSA Free

Customer Reviews for EIF3C Antibody - BSA Free (1)

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  • EIF3C Antibody
    Name: Hyun Yong Jin
    Application: Western Blot
    Sample Tested: B cells
    Species: Mouse
    Verified Customer | Posted 02/28/2017
    eIF3C expression in naive and activated (overnight) murine B cells.
    EIF3C Antibody - BSA Free NB100-511

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