ELMO1 Antibody

Novus Biologicals | Catalog # NB100-875

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ELMO1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence PKEPSNYDFVYDCN corresponding to C-Terminus according to NP_055615.8, NP_569709.1, NP_001034548.1.

Specificity

This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ELMO1 Antibody

Western Blot: ELMO1 Antibody [NB100-875]

Western Blot: ELMO1 Antibody [NB100-875]

Western Blot: ELMO1 Antibody [NB100-875] - (0.3ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunoprecipitation: ELMO1 Antibody [NB100-875]

Immunoprecipitation: ELMO1 Antibody [NB100-875]

Immunoprecipitation: ELMO1 Antibody [NB100-875] - (0.3ug/ml) staining of Human Frontal Cortex (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ELMO1 Antibody

Application
Recommended Usage

Immunoprecipitation

1:10 - 1:500

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
Western blot: Approx 83kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8kDa according to NP_055615.8).
Immunoprecipitation: This antibody has been successfully used in IP from BAEC cells as described in the following paper: Epting et al, Circ Res. 2010 Jul 9;107(1):45-55; PMID: 20466982. IP; An anonymous customer found positive results in IP on Human promyelocytic leukemia cells (HL60)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ELMO1

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Alternate Names

ced-12 homolog 1, CED12CED-12, ELMO-1, engulfment and cell motility 1, engulfment and cell motility 1 (ced-12 homolog, C. elegans), engulfment and cell motility protein 1, KIAA0281MGC126406, Protein ced-12 homolog

Entrez Gene IDs

9844 (Human); 140580 (Mouse)

Gene Symbol

ELMO1

UniProt

NP_055615 (Human)

Additional ELMO1 Products

Product Documents for ELMO1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ELMO1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ELMO1 Antibody

There are currently no reviews for this product. Be the first to review ELMO1 Antibody and earn rewards!

Have you used ELMO1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies