epithelial Sodium Channel alpha Antibody (HL2967)

Novus Biologicals | Catalog # NBP3-48756

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2967
View all epithelial Sodium Channel alpha Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment encompassing a sequence within the Extracellular domain of human epithelial Sodium Channel alpha. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for epithelial Sodium Channel alpha Antibody (HL2967)

epithelial Sodium Channel alpha Antibody (HL2967)

Western Blot: epithelial Sodium Channel alpha Antibody (HL2967) [NBP3-48756] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with epithelial Sodium Channel alpha antibody [HL2967] (NBP3-48756) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
epithelial Sodium Channel alpha Antibody (HL2967)

Western Blot: epithelial Sodium Channel alpha Antibody (HL2967) [NBP3-48756] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with epithelial Sodium Channel alpha antibody [HL2967] (NBP3-48756) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for epithelial Sodium Channel alpha Antibody (HL2967)

Application
Recommended Usage

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: epithelial Sodium Channel alpha

Epithelial Sodium Channel alpha (SCNN1A) is a subunit of the epithelial sodium channel (ENaC). ENac has high sodium selectivity, low conductance, and amiloride sensitivity. The functional channel of ENaC is composed of at least 3 subunits, alpha (SCNN1A), beta (SCNN1B), and gamma (SCNN1G). The 3 subunits show sequence similarities to one another, indicating descent from a common ancestral gene. Each encodes a protein containing 2 transmembrane domains, with intracellular amino and carboxyl termini. The alpha subunit supports sodium conductance when expressed alone; the beta and gamma subunits do not support sodium conductance by themselves, but greatly augment the channel activity when expressed in conjunction with the alpha subunit. ENaC in the kidney, lung and colon plays an essential role in trans- epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium re-uptake in the distal nephron of the kidney, thus regulating blood pressure. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddel's syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1).

Long Name

Amiloride-sensitive sodium channel subunit alpha

Alternate Names

Alpha-ENaC, Alpha-NaCH, ENaCA, SCNEA, SCNN1, SCNN1A

Gene Symbol

SCNN1A

Additional epithelial Sodium Channel alpha Products

Product Documents for epithelial Sodium Channel alpha Antibody (HL2967)

Certificate of Analysis

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Product Specific Notices for epithelial Sodium Channel alpha Antibody (HL2967)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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