ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody

Novus Biologicals | Catalog # NBP3-13232

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1221/Tyr1222 of human ErbB2/Her2. The exact sequence is proprietary.

Modification

p Tyr 1221, p Tyr 1222

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody

Western Blot: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232]

Western Blot: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232]

Western Blot: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1221/Tyr1222) antibody (NBP3-13232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody

Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232] -

Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody detects Her2 / ErbB2 (phospho Tyr1221/Tyr1222) protein at cell membrane by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).
Sample: Paraffin-embedded human breast carcinoma.
Her2 / ErbB2 (phospho Tyr1221/Tyr1222) stained by Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody (NBP3-13232) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ErbB2/Her2

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody

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  • Q: I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?

    A: A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

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