ERK1/ERK2 [p Thr185, p Tyr187] Antibody

Novus Biologicals | Catalog # NBP3-13137

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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ERK1/ERK2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr202/Tyr204 of human ERK1/ERK2. The exact sequence is proprietary.

Modification

p Thr 185, p Tyr 187

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for ERK1/ERK2 [p Thr185, p Tyr187] Antibody

Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137]

Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137]

Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ERK1 (phospho Thr202/Tyr204) / ERK2 (phospho Thr185/Tyr187) antibody (NBP3-13137) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137]

Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137]

Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) / ERK2 (phospho Thr185/Tyr187) antibody (NBP3-13137) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137]

Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137]

Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) / ERK2 (phospho Thr185/Tyr187) antibody (NBP3-13137) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for ERK1/ERK2 [p Thr185, p Tyr187] Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERK1/ERK2

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Long Name

Extracellular Signal-regulated Kinase 1/2

Alternate Names

ERK1, ERK-1, ERT2, Extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAPK 1, Microtubule-associated protein 2 kinase, mitogen-activated protein kinase 3, p44erk1, p44-ERK1, p44mapk, p44-MAPK, PRKM3

Gene Symbol

MAPK3

Additional ERK1/ERK2 Products

Product Documents for ERK1/ERK2 [p Thr185, p Tyr187] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ERK1/ERK2 [p Thr185, p Tyr187] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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