FAI

Key Product Details

Product Description

FAI is a reagent for RNA SHAPE-MaP experiments in live cells. Can be used to map RNA structures in vivo by forming stable 2'-OH adducts with RNA, blocking reverse transcriptase elongation. In vitro, FAI activity can be quenched with Dithiothreitol (Cat. No. 3154) and can be used to probe the secondary structure of mouse embryonic stem cell 5S rRNA. At elevated temperatures, FAI forms more stable adducts and exhibits less hydrolysis than NAI (Cat. No 7004).

Product Specifications for FAI

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for FAI

The following data is based on the product molecular weight 176.17.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	5.68 mL	28.38 mL	56.76 mL
5 mM	1.14 mL	5.68 mL	11.35 mL
10 mM	0.57 mL	2.84 mL	5.68 mL
50 mM	0.11 mL	0.57 mL	1.14 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Spitale RNA SHAPE analysis in living cells. Nat.Chem.Biol. 2013 PMID: 23178934
• Chan Comparative analysis reveals furoyl in vivo selective hydroxyl acylation analyzed by primer extension reagents form stable ribosyl ester adducts. Biochemistry 2017 PMID: 28319368

Product Documents for FAI