FBXW12 Antibody (1A9) - Azide and BSA Free

Novus Biologicals | Catalog # H00285231-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A9

Format

Azide and BSA Free
View all FBXW12 Primary Antibodies »

Product Specifications

Immunogen

FBXW12 (NP_996985.1, 265 a.a. ~ 354 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LLRPSEGSDPLSTFLPHKLCASACWTPKVKNRITLMSQSSTGKKTEFITFDLTTKKTGGQTVIQAYEIASFQVAAHLKCPIWMGASDGYM

Specificity

F-box and WD repeat domain containing 12

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for FBXW12 Antibody (1A9) - Azide and BSA Free

Western Blot: FBXW12 Antibody (1A9) [H00285231-M03]

Western Blot: FBXW12 Antibody (1A9) [H00285231-M03]

Western Blot: FBXW12 Antibody (1A9) [H00285231-M03] - Analysis of FBXW12 expression in HepG2.
Sandwich ELISA: FBXW12 Antibody (1A9) [H00285231-M03] - Detection limit for recombinant GST tagged FBXW12 is 3 ng/ml as a capture antibody.

Applications for FBXW12 Antibody (1A9) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FBXW12

Members of the F-box protein family, such as FBXW12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603034), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM]

Alternate Names

F-box and WD repeat domain containing 12, F-box and WD-40 domain-containing protein 12, F-box- and WD40-repeat-containing protein, F-box only protein 35MGC120386, F-box/WD repeat-containing protein 12, Fbw12, FBW12MGC120387, FBXO12FBXO35F-box and WD-40 domain protein 12, MGC120385

Entrez Gene IDs

285231 (Human)

Gene Symbol

FBXW12

UniProt

NP_996985 (Human)

Additional FBXW12 Products

Product Documents for FBXW12 Antibody (1A9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FBXW12 Antibody (1A9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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