Fibroblast Activation Protein alpha/FAP Antibody (1E5) - Azide and BSA Free

Novus Biologicals | Catalog # H00002191-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E5

Format

Azide and BSA Free
View all Fibroblast Activation Protein alpha/FAP Primary Antibodies »

Product Specifications

Immunogen

FAP (AAH26250, 525 a.a. ~ 624 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPQFDRSKKYPLLIQVYGGPCSQSVRSVFAVNWISYLASKEGMVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWS

Specificity

FAP - fibroblast activation protein, alpha

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01]

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01]

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Analysis of FAP expression in transfected 293T cell line by FAP monoclonal antibody (M01), clone 1E5.Lane 1: FAP transfected lysate(87.7 KDa).Lane 2: Non-transfected lysate.
Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01]

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01]

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - FAP monoclonal antibody (M01), clone 1E5 Analysis of FAP expression in HepG2.
Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01]

Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01]

Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Analysis of FAP transfected lysate using anti-FAP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FAP MaxPab rabbit polyclonal antibody.
ELISA: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01]

ELISA: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01]

ELISA: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Detection limit for recombinant GST tagged FAP is approximately 0.1ng/ml as a capture antibody.

Applications

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reviewed Applications

Read 1 review rated 5 using H00002191-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fibroblast Activation Protein alpha/FAP

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.

Alternate Names

FAP, Seprase

Entrez Gene IDs

2191 (Human)

Gene Symbol

FAP

OMIM

600403 (Human)

UniProt

AAH26250 (Human)

Additional Fibroblast Activation Protein alpha/FAP Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fibroblast Activation Protein alpha/FAP Antibody (1E5) - Azide and BSA Free

Customer Reviews (1)

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  • Name: Christian Zuppinger
    Application: Western Blot
    Sample Tested: lysates of 2D standard cultures of primary cardiac fibroblasts, RIPA or Laemmli lysate buffer was used
    Species: Human
    Verified Customer | Posted 07/14/2016
    WB of primary human cardiac fibroblasts lysates
    Fibroblast Activation Protein alpha/FAP Antibody (1E5) - Azide and BSA Free H00002191-M01

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