Ficolins-1 (M-ficolin), -2, and -3 are secreted pattern-recognition lectins that contribute to the innate immune recognition of pathogens. Ficolins have one fibrinogen-like domain and one collagen-like domain and circulate as disulfide-linked homo-oligomers. Following ligand binding, ficolins associate with MASP serine proteases to trigger the lectin complement cascade.
Ficolin-3 Antibody (FCN334) [DyLight 755]
Novus Biologicals | Catalog # NBP1-41283IR
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Immunoprecipitation
Label
DyLight 755 (Excitation = 754 nm, Emission = 776 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # FCN334
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Product Specifications
Immunogen
Recombinant human H-ficolin
Reactivity Notes
Human.
Specificity
Reacts specifically with H-ficolin. No cross-reaction has been observed with L- or M-ficolin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for Ficolin-3 Antibody (FCN334) [DyLight 755]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP1-41283. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Ficolin-3
Long Name
Ficolin [Collagen/Fibrinogen Domain Containing] 3
Alternate Names
FCN3, FCNH, Ficolin3, H-Ficolin, HAKA1
Gene Symbol
FCN3
Additional Ficolin-3 Products
Product Documents for Ficolin-3 Antibody (FCN334) [DyLight 755]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Ficolin-3 Antibody (FCN334) [DyLight 755]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Immunoprecipitation Protocol
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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