Key Product Details

Species Reactivity

Bacteria

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 06

Format

Azide and BSA Free
View all Flagellin Primary Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Listeria monocytogenes Flagellin (Accession#: YP_013330.1; Met1-Ser287).

Reactivity Notes

Reacts with Listeria monocytogenes

No cross-reactivity in ELISA with:

E. coli cell lysate

Specificity

No cross-reactivity in ELISA with: E. coli cell lysate

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Flagellin Antibody (06) - Azide and BSA Free

Western Blot: Flagellin Antibody (06) [NBP3-05967]

Western Blot: Flagellin Antibody (06) [NBP3-05967]

Western Blot: Flagellin Antibody (06) [NBP3-05967] - Listeria monocytogenes flagellin / flaA Recombinant Protein Lane A: 10ng. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.
Western Blot: Flagellin Antibody (06) [NBP3-05967]

Western Blot: Flagellin Antibody (06) [NBP3-05967]

Western Blot: Flagellin Antibody (06) [NBP3-05967] - Listeria monocytogenes flagellin / flaA Recombinant Protein Lane A: 200ngLane B: 50ng. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.

Applications for Flagellin Antibody (06) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:2000

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Flagellin Antibody (06) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Flagellin Antibody (06) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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