FoxP3

Human

The RetroMax system is designed for maximal virus titer in 293 cells. It takes advantages of two properties of 293 cells, i) high level of transfectibility, ii) strong E1A-mediated stimulation of CMV promoter controlled transcription. 293 cells are nonmurine in origin, hence avoiding the problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells). Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells to permit infection of more than 50% of dividing target cells in culture.
Amplify the plasmid DNA by transforming on to a suitable competent bacteria, e.g. DH5alpha or XL-1 Blue. Plate on LB agar plate containing 50ug/ml ampicillin. Start a culture from a single colony for plasmid DNA preparation.

20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20C. Avoid freeze-thaw cycles.