Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda Clone # 45

Format

BSA Free
View all Ghrelin/Obestatin Primary Antibodies »

Product Specifications

Immunogen

Synthetic human Ser3-octanoyl ghrelin-28

Epitope

Not determined. Probably includes residues 11 or 12 to account for the lower cross-reactivity with rat ghrelin

Specificity

Binds human octanoylated ghrelin and human des-acyl ghrelin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda

Description

Novus Biologicals Mouse Ghrelin/Obestatin Antibody (45) - BSA Free (NBP1-47183) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Ghrelin/Obestatin Antibody (45) - BSA Free

ELISA: Ghrelin/Obestatin Antibody (45) - BSA Free [NBP1-47183]

ELISA: Ghrelin/Obestatin Antibody (45) - BSA Free [NBP1-47183]

ELISA: Ghrelin/Obestatin Antibody (45) - BSA Free [NBP1-47183] - The calibration curve of a sandwich assay for Ghrelin using NBP1-47183 as the capture antibody and NBP1-47182B as the biotinylated detection antibody.

Applications for Ghrelin/Obestatin Antibody (45) - BSA Free

Application
Recommended Usage

ELISA

1:4000
Application Notes
ELISA: NBP1-47183 binds human ghrelin in solution. NBP1-47183 (as a capture antibody) forms a pair with NBP1-47182 as a biotinylated detection antibody for measuring total human ghrelin by sandwich ELISA. WB: NBP1-47183 can be used in Western blotting.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Ghrelin/Obestatin

Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons1,2. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form."Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food may reflect those of active ghrelin3.

Alternate Names

appetite-regulating hormone, ghrelin, ghrelin/obestatin preprohormone, ghrelin/obestatin prepropeptide, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, MTLRPgrowth hormone secretagogue receptor ligand, obestatin, Protein M46

Gene Symbol

GHRL

Additional Ghrelin/Obestatin Products

Product Documents for Ghrelin/Obestatin Antibody (45) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ghrelin/Obestatin Antibody (45) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Nutrition and Appetite
Obesity

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