Glutathione S-Transferase/GST Antibody (965427)

R&D Systems | Catalog # MAB9499

R&D Systems

Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 965427
View all Glutathione S-Transferase/GST Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant  Glutathione S‑Transferase/GST epitope tag
Accession # P08515

Specificity

Detects Glutathione S‑Transferase/GST in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Glutathione S-Transferase/GST Antibody (965427)

Detection of Glutathione S-Transferase/GST antibody by Western Blot.

Detection of Glutathione S‑Transferase/GST by Western Blot.

Western blot shows lysates of GST tagged Ubiquitin, GST tagged ISG-15, GST tagged FLAG®, HEK293T human embryonic kidney cell line,E. colitransfected with GST tagged BRDG1-SH2, andSf21 transfected GST tagged PARK1. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Glutathione S-Transferase/GST Monoclonal Antibody (Catalog # MAB9499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S-Transferase/GST as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Glutathione S-Transferase/GST Antibody (965427)

Application
Recommended Usage

Western Blot

0.25 µg/mL
Sample: GST tagged Ubiquitin, GST tagged ISG-15, GST tagged FLAG®, HEK293T human embryonic kidney cell line, E. coli transfected with GST tagged BRDG1-SH2, and Sf 21 transfected GST tagged PARK1

Reviewed Applications

Read 1 review rated 4 using MAB9499 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glutathione S-Transferase/GST

Alternate Names

Glutathione STransferase

Entrez Gene IDs

2950 (Human)

Gene Symbol

GSTP1

UniProt

P08515

Additional Glutathione S-Transferase/GST Products

Product Documents for Glutathione S-Transferase/GST Antibody (965427)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Glutathione S-Transferase/GST Antibody (965427)

For research use only

Customer Reviews for Glutathione S-Transferase/GST Antibody (965427) (1)

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  • Glutathione S-Transferase/GST Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 01/25/2018
    Glutathione S-Transferase/GST Antibody (965427) MAB9499

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