GM-CSFR alpha Antibody (2G5) - Azide and BSA Free

Novus Biologicals | Catalog # H00001438-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2G5

Format

Azide and BSA Free
View all GM-CSF R alpha Primary Antibodies »

Product Specifications

Immunogen

CSF2RA (AAH02635.1, 1 a.a. ~ 400 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLLLVTSLLLCELPHPAFLLIPEKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTDKKNRVVEPRLSNNECSCTFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARGPTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREIGIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKNTQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFGSDDGNLGSVYIYVLLIVGTLVCGIVLGFLFKRFLRIQRLFPPVPQIKDKLNDNHEVEDEIIWEEFTPEEGKGYREEVLTVKEIT

Specificity

CSF2RA - colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) (2G5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GM-CSFR alpha Antibody (2G5) - Azide and BSA Free

Western Blot: GM-CSFR alpha Antibody (2G5) [H00001438-M03]

Western Blot: GM-CSFR alpha Antibody (2G5) [H00001438-M03]

Western Blot: GM-CSFR alpha Antibody (2G5) [H00001438-M03] - Analysis of CSF2RA expression in transfected 293T cell line by CSF2RA monoclonal antibody (M03), clone 2G5. Lane 1: CSF2RA transfected lysatE (46.2 KDa). Lane 2: Non-transfected lysate.
Proximity Ligation Assay: GM-CSFR alpha Antibody (2G5) [H00001438-M03]

Proximity Ligation Assay: GM-CSFR alpha Antibody (2G5) [H00001438-M03]

Proximity Ligation Assay: GM-CSFR alpha Antibody (2G5) [H00001438-M03] - PProximity Ligation Analysis (PLA) of protein-protein interactions between KIT and CSF2RA. HeLa cells were stained with anti-KIT rabbit purified polyclonal 1:1200 and anti-CSF2RA mouse monoclonal antibody 1:50. Signals were detected by Duolink (R) 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.represents the detection of protein-protein interaction complex.
ELISA: GM-CSFR alpha Antibody (2G5) [H00001438-M03]

ELISA: GM-CSFR alpha Antibody (2G5) [H00001438-M03]

ELISA: GM-CSFR alpha Antibody (2G5) [H00001438-M03] - Detection limit for recombinant GST tagged CSF2RA is 3 ng/ml as a capture antibody.

Applications for GM-CSFR alpha Antibody (2G5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GM-CSF R alpha

The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble. [provided by RefSeq]

Long Name

Granulocyte Macrophage Growth Factor Receptor alpha

Alternate Names

CD116, CSF2R, CSF2RA, CSF2RY, GM-CSFRa, GMCSFR alpha

Entrez Gene IDs

1438 (Human)

Gene Symbol

CSF2RA

UniProt

AAH02635 (Human)

Additional GM-CSF R alpha Products

Product Documents for GM-CSFR alpha Antibody (2G5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GM-CSFR alpha Antibody (2G5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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