Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free

Novus Biologicals | Catalog # NBP3-43581

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1G8

Format

BSA Free
View all Hemoglobin beta Primary Antibodies »

Product Specifications

Immunogen

The original antibody was generated by immunizing BALB/c mice with synthetic N-terminal glycopeptide (VHLTPEC) and KLH conjugate. (Uniprot# P68871)

Specificity

This antibody binds human hemoglobin glycated at the N-terminal valine of the hemoglobin beta chain and does not cross react with non-glycated hemoglobin.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This is a chimeric antibody made using the variable domain sequences of the original Mouse IgG2a to improve compatibility with existing reagents, assays, and techniques.

Scientific Data Images for Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free

Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free

Western Blot: Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free [NBP3-43581] -

Western Blot: Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free [NBP3-43581] - Human liver (A) (0.01ug/ml), heart (B) (0.01ug/ml), placenta (C) (0.01ug/ml), and lungs (D) (0.003ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with NBP3-43581 at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.

Applications for Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hemoglobin beta

The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.

Alternate Names

beta globin chain, beta-globin, CD113t-C, HBBB, HBD, Hemoglobin beta chain, hemoglobin subunit beta, hemoglobin, beta

Gene Symbol

HBB

Additional Hemoglobin beta Products

Product Documents for Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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